莱茵衣藻玻璃珠法快速转化检测体系的建立  

Construction of a convenient method for Chlamydomonas reinhardtii glass-bead transformation assay

作　　者：黄非[1] 黄秦[1] 黄敏[1] 易秀丽[1] 郑红波[1] 乔代蓉[1] 曹毅[1] 

机构地区：[1]四川大生命科学学院四川省生物信息与代谢工程共享实验平台,成都610064

出　　处：《四川大学学报（自然科学版）》2008年第3期694-698,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30500287;30671125;30470928);四川省杰出青年基金(05ZQ026-029)

摘　　要：为了建立在衣藻中快速简便研究外源基因功能的方法,采用玻璃珠转化法将pBI221质粒(35S,Amp,GUS)高效转化至细胞壁缺陷型衣藻品系CW-15(Arg-),通过对转化条件及GUS表达检测条件摸索,发现35S启动子在CW-15中能有效启动GUS表达,且GUS最佳检测时间约为转化后24h,重组细胞先染色后制片更有利于GUS检测.In order to find a way of studying the heterogenous nuclear segments transformed into Chlamydornonas reinhardtii, pBI221 was transformed into Chlarnydomonas reinhardtii CW-15, without Arg. It is showed that 35S promoter could naturally cause GUS expression. The best time point of GUS assay was about 24 hours after transformation. Transformant was stained before rooting cells on vitreous slice. It is better than Jefferson＇ protocol.

关 键 词：莱茵衣藻 转化 GUS 瞬时表达 

分 类 号：Q78[生物学—分子生物学]