HPLC测定覆盆子中没食子酸含量  

Quantitative Determination of Gallic Acid in Fructus Rubi by HPLC

作　　者：李天傲[1] 谭喜莹[2] 

机构地区：[1]浙江大学城市学院医学与生命科学院,杭州310015 [2]江苏省中医院,南京210029

出　　处：《中国现代应用药学》2008年第3期235-237,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的用高效液相色谱法测定覆盆子中没食子酸的含量。方法采用Alltech C18柱（250mm×4．6mm，5μm），流动相为甲醇-0．4％磷酸水溶液（10：90），流速1．0mL·min^-1，检测波长278nm，柱温室温。结果没食子酸在5．24～104．80μg·mL。浓度范围呈良好的线性关系，r=0．9992，平均回收率为97．6％，RSD为1．9％。结论本法稳定性、重现性好，可作为覆盆子药材质量控制的依据之一。OBJECTIVE To determine the content of gallic acid in Fructus Rubi by HPLC. METHODS The analysis was carried out on C18 column （250mm × 4.6mm,5 μm）. Mobile phase was MeOH-0.4% H3 PO4 （ 10：90 ） , flow rate was 1.0 mL · min^ - 1 , and the wavelength was set at 278 nm. RESULTS The linearity of gallic acid was in the range of 5.24 - 104.80 μg · mL^- 1 （ r = 0. 999 2） , and the average recovery was 97.6% , RSD = 1.9%. CONCLUSION This method is simple, sensitive and accurate to determine the content of gallic acid in Fructus Rubi.

关 键 词：覆盆子 没食子酸 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学] R287[医药卫生—药学]