藏红花素对人膀胱移行细胞癌T24细胞裸鼠移植瘤的实验研究  

EFFECT OF CROCIN ON XENOGRAFTED TUMOR OF HUMAN BLADDER CANCER T24 CELL LINE ON NUDE MICE

作　　者：赵培[1] 罗春丽[1] 吴小候[2] 胡宏波[1] 吕纯芳[1] 冀慧莹[1] 

机构地区：[1]重庆医科大学医学检验系,临床检验诊断学省部共建教育部重点实验室,重庆市重点实验室 [2]重庆医科大学附属第一医院泌尿科,重庆400016

出　　处：《中国组织化学与细胞化学杂志》2008年第3期308-312,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：重庆市科委自然科学基金[2005BB5309]

摘　　要：目的检测藏红花素(crocin)对人膀胱移行细胞癌T24细胞株裸鼠移植瘤的作用。方法12只裸鼠右后腿背侧皮下接种T24移植瘤细胞后随机分为PBS对照组(6只)和50mmol/L藏红花素治疗组(6只)。从接种后第28d开始,每3d一次于肿瘤局部注射给予PBS或藏红花素,每3d测量1次肿瘤大小,并绘制移植瘤生长曲线;处理后第21d杀鼠摘取移植瘤,光镜、电镜观察肿瘤组织形态改变;RT-PCR检测Bcl-2、Bax mRNA表达;SP-免疫组织化学法分析sur-vivin、cyclinD1蛋白表达。结果藏红花素对人膀胱癌T24细胞裸鼠移植瘤的生长具有抑制作用,抑制率达28·7%。HE染色结果显示肿瘤标本内出现片状坏死灶;电镜观察可见凋亡的形态学改变;RT-PCR检测结果显示Bcl-2的表达水平下降,而Bax的表达水平上调;免疫组化结果显示经藏红花素处理后survivin、CyclinD1的表达下调。结论藏红花素能够抑制人膀胱癌T24细胞裸鼠移植瘤的生长,作用的分子机制与下调Bcl-2、survivin、cyclinD1和上调Bax基因表达有关。Objective To investigate the effects of Crocin on the xenografted tumor of the human bladder cancer T24 cell line on nude mice. Methods Twelve nude mice which had been inoculated with T24 cells by subcutaneous injection into the right legs were divided into the PBS control group （6 mice） and the 50mmol/L Crocin group （6 mice） . They were given PBS or Crocin into the tumor once every 3 days, starting on the 28^th day after tumor cell transplantation. Tumor volumes were measured every 3 days for drawing the growth curve. Animals were executed on the 21^th day after treatment. Microscopy and electron microscopy were used to observe the morphological changes. RT-PCR was used to detect Bcl-2 and Bax mRNA expressions. The expressions of survivin and cyclin D1 were detected by immunohistochemistry. Results Crocin inhibited the growth of xenograft tumor, and the inhibitory rate was 28. 7%. Local necroses were observed in the tumor specimens by HE staining. Distinct morphological changes of cell apoptosis were observed by electron microscopy. The Bcl-2 expression determined by RT-PCR was down-regulated, but the Bax expression up-regulated, cyclin D1 and survivin expressions determined by immunohistochemical staining were down-regulated after Crocin treatment. Conclusion Crocin can inhibit the growth of xenografted tumor of the human bladder cancer T24 cell line on nude mice. The mechanism may be down-regulating the expressions of Bcl-2, survivin, and cyclin D1 and up-regulating the expression of Bax.

关 键 词：藏红花素 膀胱癌 裸鼠 Bcl-2 BAX SURVIVIN CYCLIND1 

分 类 号：R737.14[医药卫生—肿瘤]