磷酸化p38在非小细胞肺癌中表达的意义  

Expression of Activated p38 in Non-small Cell Lung Cancer and Their Clinical Significance

作　　者：王志强[1] 谢宗涛[1] 陈丽达[2] 汪炳华[2] 蔡铭[1] 

机构地区：[1]江苏无锡市第四人民医院胸心外科苏州大学附属第四医院胸心外科,214062 [2]武汉大学医学院生物化学与分子生物学系

出　　处：《肿瘤防治研究》2008年第6期406-407,410,共3页Cancer Research on Prevention and Treatment

摘　　要：目的探讨丝裂原活化蛋白激酶p38在非小细胞肺癌组织中的表达与活性,以及它与肺癌临床病理特征之间的相关性。方法应用固定化蛋白质印迹法检测52例非小细胞肺癌及癌旁正常肺组织中p38和磷酸化p38(P-p38)的表达情况;免疫组织化学法分析其在细胞内的定位。结果肺癌组织中p38的表达水平与癌旁正常组织无明显差别(P>0.05);而所有癌组织标本中P-p38的表达水平全部增高,为癌旁组织的2.1倍(P<0.01);P-p38的表达在鳞癌、腺癌组织中无明显差异,与肺癌肿瘤直径大小、淋巴结转移及TNM分期无关。免疫组织化学显示p38分布在胞浆内,而P-p38在胞浆和胞核内均有表达。结论p38的过度激活可能在肺癌的发生、发展过程中起着重要作用。Objective To investigate the expression and activation of mitogen-activated protein kinase p38 and its relationship with clinicopathological characters in nonsmall cell lung cancer. Methods Samples were obtained from 52 patients with non small cell lung cancer. Western blot was used to measure the activation and expression of p38 and P-p38. Immunohistochemistry was used for localization of p38 and Pp38. Results In all 52 samples,the expression level of P-p38 in lung cancer tissues increased,which was 2. 1 times as high as compared with those in adjacent normal tissues（P〈0. 01 ）, but there were no difference of the level of p38. The expression level of P-p38 was found no correlativity with tumor size,TNM staging,lymph node metastasis and pathologic type. Immunohistochemistry showed that P-p38 was located in both nucleus and cytoplasm, and p38 was found only in cytoplasm. Conclusion The overactivity of p38 may play an important role in the development of human non-small cell lung cancer.

关 键 词：肺癌 P38 固定化蛋白质印迹 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]