筛选转基因动物的新方法——PCR及其产物测序法  

A New Method of Screening Transgenic Animal: PCR and Its Products Sequencing.

作　　者：何泉[1] 李鹏[1] 姜立群[1] 陈兰英[1] 

机构地区：[1]中国医学科学院阜外心血管病医院中国协和医科大学心血管病研究所,北京100037

出　　处：《生物化学与生物物理进展》1997年第6期552-554,共3页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金!(395 70 2 97);中国医学科学院基金资助课题

摘　　要：用特异性引物对肌球蛋白轻链 2启动子 (myosinlightchain 2 ,MLC2 ) 糜酶融合基因的转基因新生鼠鼠尾DNA进行PCR筛选 ,低熔点琼脂糖凝胶电泳回收阳性样品PCR所扩出的DNA条带 ,纯化后用同一对引物中的一个进行单引物PCR测序 ,与所转外源基因序列比较 ,进一步确定整合有外源基因的阳性鼠 .PCR及PCR产物测序法检测转基因动物具有操作方便 。The primary detection of the MLC 2 chymase fusion gene transgenic mice was carried out by means of polymerase chain reaction(PCR) with two specific primers, which span across MLC2 promoter region and chymase structure gene region. The DNA electrophoretic bands of PCR products were recovered and purified , then sequenced by PCR sequencing method with one of the two primers. Final determination of the transgenic mice was conducted through comparing the sequencing results with sequences of transfered gene. This method is convenient, effective and specific.

关 键 词：基因工程 转基因动物 筛选 聚合酶链反应 测序法 

分 类 号：Q789[生物学—分子生物学]