大肠杆菌不同菌株基因组DNA的多态性分析  

DNA POLYMORPHISM IN THE GENOMES OF DIFFERENT ESCHERICH1A COLI STRAINS

作　　者：王瑛[1] 郭永志[1] 沈孝宙[1] Leung F.C. 

机构地区：[1]中国科学院动物研究所,北京100080 [2]香港大学动物学系

出　　处：《微生物学报》1997年第6期455-462,共8页Acta Microbiologica Sinica

摘　　要：从大肠杆菌K12菌株JM109基因组克隆了两段DNA重复序列,长度为0.9和0.6kb,分别命名为ECR-1和ECR-6。以ECR-1和ECR-6重复序列作DNA多态性分析的探针,可以鉴别大肠杆菌非常相近的菌株。表明ECR-1和ECR-6 DNA序列可用于大肠杆菌菌株的分类、流行病学和微生态学研究以及大肠杆菌各种致病菌株的临床诊断。Bacteria have been traditionally classified on the basis of their morphology, biochemical reaction, serology and etc. However, in some case these methods could not authenticate the closely related bacteria strains. In this study, we cloned two repetitive DNA sequences with 0.9 and 0.6 kb in length from Escherichia coli K12 strain JM109 and designated as ECR-1 and ECR-6 respectively. Using ECR-1 and ECR-6 sequences or their combination as the probes for DNA polymorphism analysis, we were be able to develop a molecular method of biotyping for the identification of very closely related strains of Escherichia coli. Both of ECR-1 and ECR-6 probes could be applied for the taxonomy, epidemiological and microecological studies, and clinical diagnosis for pathogenic Escherichia coli strains.

关 键 词：大肠杆菌 基因组 DNA 多态性 

分 类 号：Q939.121[生物学—微生物学] Q78