RKIP介导的ERK通路在电磁辐射致海马神经元损伤中的作用  

The role of RKIP mediated ERK pathway in hippocampus neurons injured by electromagnetic radiation

作　　者：左红艳[1] 王德文[1] 彭瑞云[1] 王水明[1] 高亚兵[1] 张志毅[2] 肖凤君[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所实验病理学研究室,北京100850 [2]军事医学科学院国家生物医学分析中心,北京100850

出　　处：《细胞与分子免疫学杂志》2008年第7期660-662,667,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：军队医药卫生基金资助项目(06Z065);国家自然科学基金资助项目(30770527)

摘　　要：目的:研究电磁辐射对原代培养海马神经元Raf激酶抑制蛋白(RKIP)和磷酸化ERK表达的影响,应用MEK的抑制剂U0126探讨RKIP介导的ERK通路在辐射损伤中的作用。方法:体外培养原代海马神经元,采用X-HPM、S-HPM及EMP作为辐射源,分别建立3种电磁辐射细胞模型。通过免疫荧光标记、激光扫描共聚焦显微镜检测RKIP和磷酸化ERK的表达;利用AnnexinV-PI双标记、流式细胞术(FCM)检测细胞凋亡与坏死率。结果:三种电磁辐射后海马神经元RKIP表达均减少,磷酸化ERK表达均增加,且胞核移位发生,辐射组间均未见明显差异;U0126预处理组较单纯辐射组神经元的凋亡与坏死率明显降低,但仍高于假辐射组。结论:RKIP介导的ERK通路过度激活是电磁辐射致海马神经元凋亡与坏死的重要机制之一,U0126对电磁辐射损伤具有一定防护作用。AIM： To study the effects of eclectromagnetic radiation on RKIP and phosphorylated ERK in primary cultured hippocampus neurons. The inhibitor of MEK U0126 was applied to investigate the role of RKIP mediated ERK pathway in radiation injury. METHODS： Primary hippocampus neurons were cultured in vitro. X-HPM, S-HPM and EMP were taken as radiation source respectively to establish three cell models exposed to electromagnetic radiation. RKIP and phosphorylated ERK were measured by immunofluorescent labelling and laser scanning confocal microscope. Apoptosis and death fraction of the cells were detected by Annexin V-PI double labelling and flow cytometry. RESULTS： After three kinds of electromagnetic radiatoon, the expression of RKIP in hippocampus neurons decreased but the expression of phosphorylated ERK increased, and its nuclear translocation occurred. No significant differences were seen between radiation groups. Apoptosis and death fraction of the neurons in U0126 pretreatment groups was significantly lower than that in radiation groups but they were still higher than those in shamradiation group. CONCLUSION： The excessive activation of RKIP mediated ERK pathway is one of the important mechanisrns for the apoptosis and death of hippocampus neurons induced by electromagetic radiation. U0126 have some protective effects on radiation injury.

关 键 词：电磁辐射 海马神经元 RKIP ERK 原代培养 

分 类 号：Q684[生物学—生物物理学]