血红素氧合酶-1及Toll样受体2/4在直肠癌组织中的表达及意义  

Expression and significance of heme oxygenase-1 and Toll-like receptor 2/4 in rectal carcinoma

作　　者：田飞[1] 张黎[1] 郭琼行[1] 崔彬[2] 张紧[3] 张淑卿[1] 邓佳[4] 

机构地区：[1]山东大学附属省立医院胃肠外科,山东济南250021 [2]山东大学附属省立医院中心实验室,山东济南250021 [3]山东大学附属省立医院病理科,山东济南250021 [4]山东大学附属省立医院病理科皮肤科,山东济南250021

出　　处：《中国现代普通外科进展》2008年第3期248-250,254,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨血红素氧合酶-1(hemeoxyenase-1,HO-1)和Toll样受体2/4(Toll-likereceptor2/4,TLR2/4)在直肠癌中的表达及意义。方法:应用免疫组织化学法和RT-PCR检测30例直肠癌组织中HO-1蛋白以及HO-1mRNA、TLR2/4mRNA的表达。结果:直肠癌组织中HO-1蛋白表达(13/30)明显高于正常组织(3/30)(P<0.05),HO-1mRNA表达也明显高于正常组织(t=15.24,P<0.05);直肠癌组织中TLR2/4mRNA的表达高于正常组织(P<0.05),且HO-1mRNA与TLR2/4mRNA表达之间存在相关性(P<0.05)。结论:HO-1和TLR2/4在直肠癌组织中高表达且呈正相关,提示参与直肠癌的发生、发展。Objective： To investigate the expression and significance of hemeoxygenase-1 （HO-1）and Toll-like receptor 2/4 （TLR2/4） in rectal carcinoma. Methods： The expression of HO-1 protein and HO-1 mRNA,TLR2/4 mRNA in 30 cases of rectal carcinoma was measured using immunohistochemical method and reverse transcriptase-polymerase chain reaction （RT-PCR）. Results： The expression of HO-1 protein in rectal carcinoma （13/30）was significantly higher than those in normal tissue（3/30）（P 〈0.05）.The expression of HO-1 mRNA was also significantly higher than those in normal tissue （t =15.24, P 〈0.05）;The expression of TLR2/4 mRNA in rectal carcinomawas higher than those in normal tissue（P 〈0.05）, and HO-1 mRNA expression was associated with TLR2/4 expression （P 〈0.05）. Conclusion： The expression of HO-1 and TLR2/4 was higher in rectal carcinoma and had association with each other.

关 键 词：直肠肿瘤 血红素氧合酶-1 TOLL样受体 

分 类 号：R735.3[医药卫生—肿瘤]