程序性死亡分子1及其配体在前房相关免疫偏离鼠虹膜睫状体中的表达  

Expression of programmed death 1 and its ligands in iris-ciliary body from mice with anterior chamber-associated immune deviation

作　　者：孟倩丽[1] 杨培增[1] 李兵[1] 周红颜[1] 黄祥坤[1] 朱怜香 

机构地区：[1]中山大学中山眼科中心中山大学葡萄膜炎研究中心中山大学眼科学国家重点实验室, 广州510060

出　　处：《中华眼科杂志》2008年第6期558-562,共5页Chinese Journal of Ophthalmology

基　　金：广东省自然科学基金研究团队项目（粤科基办[2005]04号）;国家自然科学基金资助项目（30400487）;广东省自然科学基金博士启动项目（7301447）

摘　　要：目的探讨程序性死亡分子1（PD-1）及其配体在前房相关免疫偏离（ACAID）小鼠虹膜睫状体中的表达及其意义。方法为实验研究。96只正常BALB／c小鼠随机分配建立ACAID组、阳性对照组、阴性对照组及磷酸盐缓冲液对照组，每组均为24只小鼠。通过观察迟发型超敏反应，评价ACAID的形成与否。采用荧光定量实时聚合酶链反应、免疫组织化学法检测4组小鼠虹膜睫状体中PD-1及其配体1（PD-L1）和配体2（PD-L2）mRNA和蛋白的表达。所有实验结果数据的统计学检验均采用方差分析方法。结果所有ACAID组小鼠均未出现迟发型超敏反应（DTH），表明ACAID诱导成功。PD-1 mRNA的表达在ACAID组中显著增加，与其他3组比较差异有统计学意义（F=248．109，P〈0．05）；而PD-L1 mRNA的表达在阳性对照组中显著增加（F=179．033，P〈0．05）。PD-1和PD-L1阳性细胞主要分布于虹膜根部和瞳孔缘区，多呈圆形或类椭圆形，二者的蛋白表达水平分别与它们的mRNA表达水平相一致。4组小鼠虹膜睫状体中均未检测到PD-L2 mRNA和蛋白的表达。结论ACAID小鼠虹膜睫状体中PD-1表达显著增加，可能参与了ACAID的形成。Objective To study the expression and possible implication of programmed death 1 （ PD-1 ） and its ligands in the iris-ciliary body from mice with anterior chamber-associated immune deviation （ACAID）. Methods This was an experimental study. Twenty-four BALB/c mice were divided into ACAID group, negative controls, positive controls and phosphate-buffer saline controls. ACAID was evaluated by delayed-type hypersensitivity response. Expressions of PD-1 and its ligands （ PD-L1, PD-L2 ） in the irisciliary body from ACAID mice were determined by real-time polymerase chain reaction and immunohistochemical studies. Results Delayed-type hypersensitivity was not detected in ACAID group, indicating that ACAID was induced successfully. The expression of PD-1 mRNA in ACAID group was higher than that in all other groups （ F = 248. 109, P 〈 0. 05 ）. The positive controls showed a high expression of PD-L1 mRNA （ F = 179. 033, P 〈 0.05 ）. PD-1 and PD-L1 positive cells with round or oval shapes were found in whole iris-ciliary body, especially in the iris base and pupil margin. Expression of PD-1 and PD-L1 proteins were similar to their expression at mRNA levels. Neither mRNA nor protein of PD-L2 was detected in the iris-ciliary body in all groups. Conclusion An increased expression of PD-1 in the iris-ciliary body of ACAID mice indicates that this is involved in the induction of ACAID.

关 键 词：抗原 表面 细胞凋亡调节蛋白质类 膜糖蛋白类 免疫 主动 超敏反应 迟发型 

分 类 号：R686[医药卫生—骨科学]