胃癌NET-1、Ki-67表达及意义  

Overexpression of NET-1 and Ki-67 and their significance in gastric carcinomas

作　　者：钱铮[1] 陈莉[2] 王桂兰[2] 鄂群[2] 

机构地区：[1]江苏省海门市人民医院病理科,海门226100 [2]南通大学医学院病理教研室,南通226001

出　　处：《临床与实验病理学杂志》2008年第3期305-308,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：江苏省教育厅高新技术资助项目(JHO2-118);南通市社会发展项目(s1001)

摘　　要：目的研究胃癌组织中NET-1和Ki-67表达与其预后的关系。方法分别应用免疫组化EnVision两步法检测86例胃癌组织中NET-1和Ki-67的表达,对两指标均阳性病例行免疫组化双标双染方法,原位检测两指标的表达特点和比邻关系。所有病例随访60月以上。结果胃癌组织中NET-1和Ki-67高表达分别为56.98%和74.42%,两指标阳性表达呈正相关(P<0.01)。两指标高表达分别与癌分化呈负相关(均P<0.01);与癌临床分期呈正相关(均P<0.05);与患者生存率呈负相关(均P<0.05),其3年、5年生存率均低于相应的低表达组或阴性组(P<0.05,P<0.01);NET-1表达还与癌淋巴结转移呈正相关(P<0.01)。结论NET-1、Ki-67基因蛋白高表达反映胃癌细胞群体增殖活性,上调癌进展,预后较差。Purpose To investigate the overexpression of NET-1 and Ki-67 and their prognostic significance in gastric carcinomas. Methods The overexpression of NET-1 and Ki-67 Protein was detected in paraffin-embeded sections of 86 cases of gastric carcinomas by immunohistochemical methods （EnVision）. Immunohistochemical dual color staining procedure was used to demonstrate the dual positive cases with NET-1 and Ki-67 and to show the adjacent relationship between NET-1 and Ki-67 on one section. All cases were followed up over 60 months. Results The overexpression rates of NET-1 and Ki-67 in gastric carcinomas were 56.98% and 74.42%, respectively. There was significantly positive correlation between the dual markers （P 〈0. 05）. The overexpression of NET-1 and Ki-67 was negatively correlated with carcinoma differentiation （P 〈 0. 01 ） and positively correlated with tumor clinical stages（ P 〈0. 05 ）but negatively correlated with tumor survival rates （P 〈 0. 05 ）. The survival rates within 3 and 5 years were significantly shorter in the overexpression groups of NET-1 and Ki-67 than that in low expression groups and the negative groups（P 〈0. 05 ,P 〈0. 01 ）, respectively. In addition, the overexpression of NET-1 was also positively correlated with lymph node status of tumor （P 〈0. 01 ）. Conclusions The overexpression of NET- 1 and Ki-67 gene reflects the activity of cell clonal proliferation and significantly upregulates the carcinogenesis, which accelerates the development and progression of gastric carcinomas.

关 键 词：胃肿瘤 NET-1 KI-67 预后 

分 类 号：R735[医药卫生—肿瘤]