联合检测D2-40、calretinin及HBME-1在浆膜腔积液细胞学鉴别诊断中的意义  

Combined detection of D2-40,calretinin and HBME-1 in differential diagnosis of body cavity effusion cytology

作　　者：陈颖[1] 石园[1] 孙路得[1] 谢红仙[1] 宿杰.阿克苏[1] 周燕南[1] 谭云山[1] 

机构地区：[1]复旦大学附属中山医院病理科,上海200032

出　　处：《临床与实验病理学杂志》2008年第3期337-340,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的观察D2-40、calretinin及HBME-1在浆膜腔积液间皮和转移性癌细胞中的表达,评价其用于鉴别诊断的价值。方法用细胞块技术对87例浆膜腔积液标本进行免疫组化检测,观察D2-40、calretinin和HBME-1在癌细胞和间皮细胞中的表达。结果D2-40、calretinin和HBME-1标记间皮细胞的敏感度分别为48.1%、81.0%、77.2%,特异度分别为90.2%、69.5%、61.0%。三者联合的敏感度和特异度分别为70.9%和81.7%。三者标记卵巢癌腹水间皮细胞的特异性都不高。结论D2-40、calretinin和HBME-1合用可辅助鉴别诊断转移性癌与间皮。Purpose To evaluate the value of D2-40, calretinin and HBME-1 in identifying the mesothelial cells in body cavity effusions. Methods 87 effusion specimens were assessed by immunohistochemical analysis in cell blocks. Results The sensitivity rates of D2-40, calretinin and HBME-1 were 48. 1% , 81.0% and 77.2% , respectively. The specificity rates were 90. 2% , 69.5% , 61.0%. And the combined sensitivity and specificity were 70. 9% and 81.7% , respectively. The specificity of all the three markers in identifying the mesothelial cells in ovarian cancer induced ascites was not high. Conclusion Combined detection of D2-40, calretinin and HBME-1 is an accessory method for differentiation between metastasis cancer and mesothelial cells.

关 键 词：浆膜腔积液 D240 CALRETININ HBME-1 免疫组织化学 鉴别诊断 

分 类 号：R730.4[医药卫生—肿瘤] R446.8[医药卫生—临床医学]