人脑胶质瘤中COX-2表达及其与血管新生关系  

Expression of COX-2 and its relationship to angiogenesis in human brain gliomas

作　　者：孔世奇[1] 于利洁[2] 扈玉华[1] 尹风任[1] 冀建文[1] 顾洪涛[2] 吴建梁[1] 

机构地区：[1]河北医科大学第二医院神经外科,石家庄050000 [2]河北医科大学口腔医院病理研究室,石家庄050017

出　　处：《临床与实验病理学杂志》2008年第3期341-344,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：河北省自然科学基金项目(C2006000910);河北省科技厅博士基金项目(06547009D-12)

摘　　要：目的检测COX-2、VEGF和CD34在人脑胶质瘤中的表达,探讨COX-2、VEGF与胶质瘤微血管密度及血管新生之间的关系及意义。方法用免疫组化SP法检测80例胶质瘤及10例正常脑组织中COX-2、VEGF和CD34的表达。结果COX-2与VEGF阳性细胞在坏死区周围及血管密集的区域分布密集,80例胶质瘤中二者表达的阳性率分别为68.8%和72.5%,正常脑组织中无表达;COX-2、VEGF的表达与MVD之间成正相关(r=0.927,r=0.939,P<0.05),COX-2与VEGF的表达成正相关(r=0.885,P<0.05),胶质瘤病理级别与COX-2、VEGF、MVD之间均成正相关(r=0.894,r=0.927,r=0.865,P<0.05)。结论COX-2和VEGF在胶质瘤的生长和进展过程中发挥重要作用,与其恶性度关系密切;COX-2可能通过上调VEGF的表达促进肿瘤组织血管新生。Purpose To detect the expression of cyclooxygenase-2 （ COX-2）, vascular endothelial growth factor （VEGF） and CD34 in surgically removed human gliomas and to study the relationship and significance among COX-2, VEGF, micro vessels density （MVD） and angiogenesis in gliomas. Methods Immunohistochemical SP method was used to investigate the expression of COX-2, VEGF and CD34 among 80 human glioma samples and 10 normal brain tissue samples respectively. Results Positive cells of COX-2 and VEGF were distributed in the area close to necrosis and in the area with intensive blood vessels. The positive rates of COX-2 and VEGF in 80 glioma samples were 68.8% and 72. 5% and there was no expression in normal brain tissue. The expression of COX-2 and VEGF was associated with MVD （ r = 0. 927, r = 0. 939, P 〈 0. 05 ） ; the expression of COX-2 was associated with VEGF （ r = 0. 885, P 〈 0. 05 ） ; the pathologic grade of gliomas was associated with COX-2, VEGF and MVD （r = 0. 894, r = 0. 927, r = 0. 865, P 〈0.05 ）. Conclusion COX-2 and VEGF play an important role in the glioma growing and progressing and are closely associated with malignancy. COX-2 may accelerate the tumor＇ s angiogenesis through up-regulating the expression of VEGF.

关 键 词：脑肿瘤 胶质瘤 血管新生 环氧化酶-2 血管内皮生长因子 

分 类 号：R739.41[医药卫生—肿瘤] R345[医药卫生—临床医学]