SonoVue微泡瞬时均一性对新型绒癌细胞靶向造影剂结合特性的影响  

Impact of Instantaneous Uniformity of SonoVue Microbubbles on Binding Characteristics of A New Contrast Agent Targeted to Choriocarcinoma Cells In Vitro

作　　者：周力学[1] 丁红[1] 贾彩霞[1] 李艳[2] 魏青[3] 

机构地区：[1]中山大学附属第二医院妇产科,广东广州510120 [2]广州市花都区人民医院妇产科,广东广州510800 [3]中山大学附属第二医院医研中心,广东广州510120

出　　处：《癌症》2008年第7期692-697,共6页Chinese Journal of Cancer

基　　金：广东省科技厅基金(No.2006B35901009)~~

摘　　要：背景与目的:超声微泡造影剂是目前的研究热点,通过其可提高超声扫描对肿瘤血管的检出率,但缺乏组织特异性。本研究目的是探讨在制备靶向造影剂之前SonoVue微泡瞬时均一性对绒癌细胞(choriocarcinoma cell,JAR)靶向造影剂的体外结合特性、结合率及稳定性的影响,为临床研究肿瘤细胞抗原的超声靶向定位显像、提高肿瘤的早期诊断率奠定基础。方法:研究分为微泡不均匀组(n=10),微泡均匀组(n=10),小微泡组(n=10)3组;主要检测花环形成率、流式细胞计数及花环结合形态等。用均一性不同的SonoVue微泡与兔抗人绒毛膜促性腺激素(human chorionic gonadotrophin,HCG)抗体作用制成靶向造影剂,然后分别与绒癌细胞作用,比较各组的结合率及冲洗前后的结合率。结果:各组中不均一组的靶向造影剂微泡与绒癌细胞的结合率为(60.4±1.5)%,低于微泡均匀组及小微泡组[(84.3±5.5)%和(90.6±6.8)%],差异有统计学意义(P<0.05)。冲洗前后比较,微泡均匀组靶向微泡与贴壁生长的绒癌细胞结合率变化最小,冲洗后结合率为(82.4±3.7)%(P>0.05)。流式细胞检测结果显示:微泡不均匀组、微泡均匀组、小微泡组悬液中结合了荧光标记的靶向造影剂微泡与绒癌细胞的结合率分别为72.9%、81.03%、88.5%,3组间差异有统计学意义(P<0.05)。结论:在体外制备靶向造影剂之前,SonoVue微泡瞬时均一性对绒癌细胞靶向造影剂微泡与绒癌细胞特异性结合能力有明显影响,而SonoVue微泡的均一性与适度振荡有关,SonoVue微泡瞬时不均一性直接影响靶向特异性结合率的数量。通过改善SonoVue微泡的均一性可望提高靶向造影剂结合率及靶向微泡稳定性,改善显影。BACKGROND ＆ OBJECTIVE： At present, the investigation of microbubble contrast agents is a hot spot. Although these contrast agents can increase the ultrasound detection rate of tumor vessels, they lack tissue specificity. This study was to evaluate the impact of instantaneous uniformity of SonoVue microbubbles on binding characteristics, including the adhesion rate and stability, of a new contrast agent targeted to choriocarcinoma cells （JARs） in vitro, in order to establish a foundation to explore targeted ultrasound imaging for localization of tumor cell antigens and increase the early diagnostic rate for tumors. METHODS= The objects were divided into three groups= the uneven microbubble group （n=10）, the uniform microbubble group （n=10） and the tiny microbubble group （n=10）. The rosette formation rate was counted. JARs were calculated by flow cytometry （FCM）. The shape of the rosette was recorded. The targeted contrast agent was prepared by mixing SonoVue microbubbles of different uniformity with rabbit anti-human chorionic gonadotrophin （HCG） antibody. The binding rates of the contrast agent to JARs before and after PBS rinse were analyzed. RESULTS： The binding rate was significantlylower in the uneven microbubble group （60.4±1.5）% than in the uniform microbubble group （84.3±5.5）% and the tiny microbubble group （90.6±6.8）% （P〈0.05）. The binding rates of different microbubbles to JARs before and after PBS rinse were different. The uniform microbubbles were the most stable ones, with the binding rate of （84.3±5.5）% and （82.4±3.7）% before and after PBS rinse （P〉0.05）. The binding rates of the targeted microbubbles labeled with fluorescence to JARs were 72.9%, 81.03% and 88.5% in the uneven microbubble group, the uniform microbubble group and the tiny microbubble group, respectively （P〈0.05）. CONCLUSIONS： The binding capacity of the targeted SonoVue microbubbles to JARs is related to instantaneous uniformity of the microbubble, which is det

关 键 词：SonoVue微泡 瞬时均一性 绒癌细胞 结合率 

分 类 号：R737.33[医药卫生—肿瘤]