肾衰养真胶囊对胰岛素诱导的大鼠脂肪细胞瘦素及C/EBPα表达的影响  

Effects of shenshuai yangzhen capsule on insulin-induced leptin and C/EBPα expressions in rat adipocytes

作　　者：黄丽雯[1] 魏连波[1] 龚春水[1] 邓聪[1] 

机构地区：[1]南方医科大学珠江医院中西医结合肾病中心,广州市510280

出　　处：《实用医学杂志》2008年第11期1877-1879,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30672768);广东省自然科学基金资助项目(编号:06024428);广州市科技基金资助项目(编号:2006Z3-E5051-1);南方医科大学开发基金资助项目(编号:200601)

摘　　要：目的:探讨肾衰养真胶囊对胰岛素刺激的大鼠脂肪细胞瘦素(leptin)及瘦素基因启动子CCAAT框增强子结合蛋白(C/EBPα)mRNA表达的影响。方法:给予大鼠灌胃制备肾衰养真胶囊含药血清,应用含药血清处理经胰岛素刺激的脂肪细胞,采用放免法检测上清瘦素分泌水平,RT-PCR法检测细胞C/EBPαmRNA表达水平。结果:经胰岛素干预后脂肪细胞瘦素表达显著增加,加入肾衰养真胶囊含药血清后脂肪细胞瘦素、C/EBPα的表达显著受到抑制。结论:肾衰养真胶囊可通过抑制C/EBPα的表达从而削弱胰岛素刺激引起的瘦素的过度表达。Objective To observe the effects of shenshuai yangzhen capsule on insulin-induced leptin secretion and C/EBPα mRNA expression in rat adipocytes. Methods Rat sera containing herbs were collected after intragastricly administration with the shenshuai yangzhen capsule, Adipocytes induced by insulin were incubated with heat-inactivated pharmacologic serum, Leptin secreted in culture supematant was measured by radio immunoassay. The expression of C/ EBPα mRNA in adipocytes was detected by RT-PCR. Results Insulin increased the expression of leptin in adipocytes significantly. Shenshuai yangzhen capsule inhibited the excessive expressions of leptin and C/EBPα in adipocytes induced by insulin significantly. Conclusion Shenshuai yangzhen capsule may reduce the excessive expression of leptin through inhibition of C/EBPα expression in adipocytes induced by insulin.

关 键 词：肾功能衰竭 慢性 肾衰养真胶囊 脂肪细胞 瘦素 C/EBPΑ 

分 类 号：R285.5[医药卫生—中药学] R277.525[医药卫生—中医学]