转化生长因子-β1基因修饰神经干细胞在大鼠脑缺血模型中的试验研究  

Experimental study on treatment of cerebral ischemic-reperfusion injury with TGF-β1-transfected neural stem cells transplantation in rats

作　　者：白润涛[1] 葛朝莉[1] 韩漫夫[1] 高明勇[1] 

机构地区：[1]广东省深圳市第二人民医院神经内科,深圳518000

出　　处：《临床医学》2008年第5期105-107,共3页Clinical Medicine

基　　金：深圳市科技计划项目(JH200505310604A)

摘　　要：目的通过向大鼠脑缺血损伤区的靶向移植转化生长因子-β1(TGF-β1)基因修饰的神经干细胞,观察TGF-β1基因修饰的神经干细胞对大鼠脑缺血损伤的治疗作用。方法建立大鼠的局灶性脑缺血再灌注模型,并随机分为假手术组(A组)、磷酸盐缓冲液PBS组(B组)、神经干细胞治疗组(C组)、TGF-β1基因修饰神经干细胞组(D组),根据取样时间点的不同每组又分为3、7及14 d共三组(每组5只),手术后3、7、14 d进行神经功能评分(NSS),采用5-溴-2,-脱氧尿嘧啶核苷(BrdU)标记处于增殖状态的细胞,应用免疫组化法检测BrdU阳性细胞。结果术后3、7、14 d C组和D组NSS评分明显低于A组和B组,统计学分析差异有统计学意义(P<0.05),其中D组NSS评分较C组低,两组比较差异有统计学意义(P<0.05);C组和D组大鼠在脑缺血/再灌注后3、7、14 d均可检测到BrdU阳性细胞,BrdU阳性细胞计数显示各个时间点C组和D组阳性细胞数明显多于对照组(P<0.05)。结论在局灶性脑缺血再灌注模型大鼠损伤区注入TGF-β1基因修饰的神经干细胞,对脑缺血所致的损伤具有一定的修复作用。Objective To investigate the effect of transforming growth factor - β1 （ TGF - β1 ） - transfected neural stem cells on focal cerebral ischemia -reperfusion in rat. Methods Temporary middle cerebral artery occlusion models were established and randomly divided into sham - operated group （ A group）, phosphate buffer saline group （ B group）, neural stem cell group（ C group）, TGF - β1 - transfectecl neural stem cell group（ D group）. Above four groups again divided into 3 d、7 d and 14 d group according the different times. The neurological severity scores（NSS） were evaluated in different times. 5 -Bromo -2, -cleoxyuridine （BrdU） was applied to labeling cell proliferation, immunohistoehemistry was used to detect positive cells of BrdU. Results The NSS in C group and D group were lower than A group and B group after 3 d 、7 d and 14 d （ P 〈 0.05 ）. And the NSS in D group was lower than C group after 3 d,7 d and 14 d （P 〈0.05）. C group and D group all detected positive cells of BrdU. Positive cells of BrdU were situated in the ipsilateral cortex near the infarct boundary,subventricular area and around blood vessels in the ipsilateral cortex. The positive cells of BrdU in C group and D group were more than A group and B group （ P 〈 0. 05 ）. Conclusion TGF - β1 - transfeeted neural stem cells may reduce neural damage after transplanted into the focal cerebral ischemia- reperfusion in rats.

关 键 词：转化生长因子-Β1 神经干细胞 脑缺血/再灌注损伤 

分 类 号：R743[医药卫生—神经病学与精神病学] R575.2[医药卫生—临床医学]