利用透明颤菌血红蛋白在低氧条件下提高毕赤酵母中植酸酶的表达  

Expression of Bacterial Hemoglobin with a Low O_2-induced Promoter Improves Recombinant Phytase Production in Pichia pastoris

作　　者：唐辉桂[1] 黄火清[1] 罗会颖[1] 王亚茹[1] 杨培龙[1] 姚斌[1] 

机构地区：[1]中国农业科学院饲料研究所,北京100081

出　　处：《中国农业科技导报》2008年第3期84-89,共6页Journal of Agricultural Science and Technology

基　　金：国家863计划项目(2006AA02Z213)资助

摘　　要：为了提高毕赤酵母(Pichia pastoris)中重组植酸酶的表达,在来源于Pichia stipitis的低氧诱导启动子PsADH2控制下,进行了透明颤菌血红蛋白基因vgb在重组P.pastoris中与植酸酶的共表达研究。结果表明,PsADH2启动子在低氧条件下被诱导启动,表达的血红蛋白VHb对P.pastoris宿主细胞生长无明显影响,但使重组P.pastoris中共表达的植酸酶APPA活性提高了3倍。To improve the production of a recombinant phytase APPA in Pichia pastoris under O2 limitation, the PsADH2 promoter from Pichia stipitis was employed to control the expression of Vitreoscilla hemoglobin （VHb） encoding gene （vgb） in P. pastoris. This promoter was activated under O2 limitation in P. pastoris. The expressed VHb enhanced the phytase APPA activity to 3 fold higher than that of VHb-free ceil under O2 limitation, but no obvious effect on ceil growth was observed.

关 键 词：PsADH2启动子 透明颤菌血红蛋白VHb 低氧诱导 植酸酶 毕赤酵母 

分 类 号：Q786[生物学—分子生物学]