应用二维电泳和质谱技术筛选乳腺癌相关差异表达蛋白质的初步研究  

Initial research of screening for the differentially expressed proteins in human breast carcinoma by two-dimensional gel electrophoresis and mass spectrometry

作　　者：陈晶[1] 丁丹[2] 郭慕红[1] 王汉晋[1] 姜鹏[1] 时开网[1] 

机构地区：[1]南京医科大学附属南京第一医院普外科,江苏南京210006 [2]南京医科大学第一附属医院普外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2008年第6期747-751,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市医学科技发展项目(ZKX0405)

摘　　要：目的:通过分离并鉴定乳腺癌、癌旁和正常乳腺组织的差异表达蛋白质,以发现可能用于早期诊断的乳腺癌肿瘤标志物。方法:提取人乳腺癌、癌旁和正常乳腺组织的总蛋白质,用双向电泳分离蛋白并进行比较。选择在乳腺癌组织中明显差异表达的蛋白点,行质谱分析。结果:获得了分辨率和重复性均很好的凝胶蛋白图谱。对筛选出的在乳腺癌组织中明显差异表达的20个蛋白点,共有13个蛋白点被成功鉴定,其中在乳腺癌组织中高表达的为8个,低表达的为5个。结论:乳腺癌组织相对于癌旁、正常乳腺组织蛋白存在明显的差异,通过蛋白质组学方法筛选并鉴定出的这些蛋白质可能成为用于早期诊断和评价预后的乳腺癌标志物。Objective：To find potential molecular targets for breast cancer diagnostics by analyzing and comparing the proteomes expressed in human breast carcinoma,paracancerous tissues and normal tissues. Methods：The proteins expressed in these tissues were analyzed by the two-dimensional gel electrophoresis. The differentially expressed proteins in tumors were also analyzed by mass spectrometry（MS）. Results：Protein extracts of individual sample in each type of tissues were separated on two-dimensional gels. There were twenty differentially expressed proteins in the tissues of breast cancer. Thirteen proteins were successfully identified by MS,in which eight proteins were overexpressed in tumors and the other five proteins were underexpressed. Conclusion：Significant discrepancies in protein expression exist among breast cancer tissues,paracancerous tissues and normal tissues. Some of the differentially expressed proteins found by the proteomic approach may be potential molecular targets for breast cancer diagnosis.

关 键 词：乳腺癌 蛋白质组 二维凝胶电泳 肿瘤标志物 

分 类 号：R737.9[医药卫生—肿瘤]