碘油羟基磷灰石纳米粒对兔VX2肝肿瘤Paxillin和P130Cas表达的影响  

The effect of lipiodol-hydroxyapatite nanoparticle on the expression of Paxillin and P130Cas in rabbit VX2 tumor of liver

作　　者：李高鹏[1] 陈孝平[1] 黄志勇[1] 穆拉德[1] 孙振纲[1] 梁慧芳[1] 关键[2] 夏喜刚[1] 何前进[1] 张万广[1] 胡道予[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝脏外科中心,武汉430030 [2]华中科技大学同济医学院附属同济医院放射科,武汉430030

出　　处：《腹部外科》2008年第3期181-183,共3页Journal of Abdominal Surgery

基　　金：国家"十五"重点科技攻关基金(2004BA714B)

摘　　要：目的研究碘油羟基磷灰石纳米粒(nHAP)经肝动脉灌注治疗对兔VX2肝肿瘤转移相关基因Paxillin(桩蛋白)和P130Cas表达的影响。方法将100只新西兰白兔肝内肿瘤种植后2周,随机分为5组,每组20只,设生理盐水组(A组)、nHAP组(B组)、单纯碘油组(C组)、阿霉素碘油组(D组)和碘油nHAP组(E组)。经肝动脉灌注给药后2周,采用免疫组织化学和Western blotting检测这两个基因在蛋白水平的表达。结果两种方法结果均提示:兔VX2肝肿瘤中Paxillin呈低表达而P130Cas呈高表达。与A组相比,C组和D组残余肿瘤区的Paxillin表达降低,而E组表达则显著升高(P<0.05);与A组相比,C组和D组残余肿瘤区的P130Cas表达升高,而E组表达则显著降低(P<0.05)。结论兔VX2肝肿瘤中Paxillin的低表达和P130Cas的高表达可能与其高转移性有关。单纯碘油及阿霉素碘油经肝动脉治疗可降低Paxillin的表达,而碘油nHAP可升高其表达;单纯碘油及阿霉素碘油经肝动脉治疗可升高P130Cas的表达,而碘油nHAP可降低其表达。提示碘油nHAP可能可以抑制VX2肿瘤的转移。Objective To study the effect of lipiodol-hydroxyapatite nanoparticle on the expression of the focal adhesion-associated proteins Paxillin and P130Cas in rabbit VX2 tumor of liver. Methods One hundred New Zealand white rabbits with VX2 carcinoma implanted in the left lobes of liver for two weeks were randomly divided into 5 groups（n = 20 in each group）. Every group was infused via the hepatic artery with the physiological saline（group A）, nHAP （group B）, lipidol（group C）, ADM and Lipiodol（group D）and Lipi-nHAP（group E）. The expression of Paxillin and P130Cas was detected by using immunohistochemistry and Western blot two weeks after the treatment. Results The expression of Paxillin in groups C and D was significantly lower than in group .＆,and that in group A was significantly lower than in group E（P〈0. 05）. The expression of P130Cas in groups C and D was significantly higher than in group A,and that in group A was significantly higher than in group E（P〈 0. 05）. Conclusion The low expression of Paxillin and the high expression of P130Cas may play very important roles in the metastasis of VX2 tumor. Both the Lipidol and ADM ＋ Lipiodol can lower the expression of Paxillin,while the Lipi-nHAP can enhance its expression. In contrary, both Lipidol and ADM ＋ Lipiodol can enhance the expression of P130Cas while the Lipi-nHAP can lower its expression. These results suggest that Lipi-nHAP may inhibit the metastasis of VX2 after transcatheter arterial chemoembolization.

关 键 词：羟基磷灰石类 桩蛋白 Crk相关底物 肝肿瘤 实验性 

分 类 号：R734.2[医药卫生—肿瘤] R735.7[医药卫生—临床医学]