Purification and Characterization of a Proteolytic Enzyme from Fig Latex  

作　　者：HUANG Lu QU He-zhi ZHANG Lei DU Shan-shan YANG Shuo HAO Dong-yun WANG Xiao-ping 

机构地区：[1]Key Laboratory of Molecular Enzymology, ＆ Engineering of Ministry of Educational Jilin University, Changehun 130021, P. R. China

出　　处：《Chemical Research in Chinese Universities》2008年第3期348-352,共5页高等学校化学研究（英文版）

基　　金：the National Natural Science Foundation of China(Nos.20771030 and 20671025)

摘　　要：Ficin is an important component of plants in Ficus family such as fig latex. It is of special significance in medicine and industry because it exhibits activity throughout a wide range of temperature and pH values. In this work, we purified a component of ficin from the latex homogeneity of Shandong fig trees, and the properties of the purified ficin were studied. The current findings revealed that heavy metal ions were able to inhibit ficin, while DTT, L-cysteine, and β-ME were found to promote ficin activity. It was also observed that the half life of ficin at 65 °C was longer than 1 h and the Michaelis constant（Km） for casein hydrolyzation was determined to be 1.56 mg/mL. Our study shows that this purified ficin is a cysteine protease.Ficin is an important component of plants in Ficus family such as fig latex. It is of special significance in medicine and industry because it exhibits activity throughout a wide range of temperature and pH values. In this work, we purified a component of ficin from the latex homogeneity of Shandong fig trees, and the properties of the purified ficin were studied. The current findings revealed that heavy metal ions were able to inhibit ficin, while DTT, L-cysteine, and β-ME were found to promote ficin activity. It was also observed that the half life of ficin at 65 °C was longer than 1 h and the Michaelis constant（Km） for casein hydrolyzation was determined to be 1.56 mg/mL. Our study shows that this purified ficin is a cysteine protease.

关 键 词：PROTEASE Fig-tree latex FICIN PURIFICATION Characterization 

分 类 号：Q946.5[生物学—植物学]