温度与电磁参数协同影响胰岛素分子构象与功能的光谱学方法研究  

Study on Temperature & EMF Co-Effects on Insulin Conformation and Biological Functions by Fluorescence and Raman Spectroscopy

作　　者：严喆[1] 陈树德[1] 乔登江[1] 

机构地区：[1]华东师范大学光谱学与波谱学教育部重点实验室华东师范大学物理系,上海200062

出　　处：《光谱学与光谱分析》2008年第6期1343-1347,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50137030)资助

摘　　要：近年来,脉冲电磁辐射和弱电磁辐射的生物效应已引起人们的关注。开展了众多的病理学调查和实验研究。但是研究结果并不一致,学术界存在众多争议。由于脉冲电磁辐射和弱电磁辐射不能引起被辐照生物样品产生明显的温升效应(温升小于0.1℃),不能用电磁辐射的热效应机理来解释这类生物效应。因此人们往往称之为"非热效应"。文章应用荧光光谱和拉曼光谱方法研究脉冲电场对胰岛素分子构象变化影响的结果表明:(1)尽管这类电磁辐射不会引起被辐照生物样品的温度产生明显变化(0.1℃),但是辐照时的环境温度变化则能改变辐照实验的结果。(2)环境温度能对胰岛素分子结构中的链间二硫键,C—C键的振动等在脉冲电场作用下的变化产生明显的影响,从而对脉冲电场引发的胰岛素的构象变化产生影响。这表明对于"非热效应",表征热的量度的温度,仍是脉冲和弱电磁场辐照生物效应研究中值得关注的极重要参数。Our previous studies had suggested that the intercellular signal molecule might be an important target of electromagnetic fields. Insulin, an intercellule signal molecule, plays a critical role in transferring life information. The studies on effects of pulsed electric fields （PEF） on insulin molecule are meaningful for explaining the mechanism of biological effects of electromagnetic fields. The PEF, which we used, with its highest electric field （2 × 10^6 V · m^-1） coupled into the insulin buffer, was about 1 V · cm^-1 cm, with a repeating frequency of 50 Hz. In the present study, the changes of insulin conformation induced by PEF were studied by fluorescence spectroscopy, Insulin solution was exposed to 50 Hz PEF with different electric field intensities for 5-35 min, which caused a time-and dose-dependent decrease in fluorescence intensities of insulin. Further, insulin solution was exposed to PEF at different temperatures to investigate the effects of PEF co-operated with temperature on insulin. The results indicated that the difference in temperature （about 5 ℃） could induce conflict results, which is due to the effects of PEF co-operated with temperature rather than only to the effect of temperature. The authors calculated that the increase in temperature induced by PEF was 0. 07 ℃ （less than 0. 1 ℃）. So the effects of PEF were scarcely explained by thermal effects, it belongs to ＂non-thermal effects＂ of electric fields. So it was concluded that temperature is a considerably important factor in ＂non-thermal effects＂ of electric fields, and the ignorance of variety of temperature probably result in the contrary conelusion. Further, Raman spectroscopy was used to investigate the details of structure of insulin treated by PEF co-operated with temperature. The results of Raman spectroscopy verified the effects of PEF co-operated with temperature on insulin. And the reductions of the S--S band intensity at 510 cm^-1 , the skeletal C--C stretch band intensity at 934 cm^-1 , and t

关 键 词：脉冲电场 温度 胰岛素 荧光光谱 激光拉曼光谱 

分 类 号：Q64[生物学—生物物理学]