双波长差值法测定饲料原料中的木聚糖含＊量  

Determining Content of Xylan in the Feed Raw Material by the Dual-Wave Length Spectrophotometry Method

作　　者：樊世明[1] 高玉鹏[1] 王文杰[2] 穆淑琴[2] 李军峰[1] 王芝英[2] 郭嘉[2] 张海波[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]天津市畜牧兽医研究所,天津300112

出　　处：《西北农业学报》2008年第3期78-80,共3页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：天津市自然科学基金项目(项目编号:033608411)

摘　　要：以D-木糖为标准物质,地衣酚-盐酸为显色剂,建立了以双波长吸光度差值测定饲料原料中木聚糖含量的方法,当反应溶液中D-木糖浓度为0-100μg/mL时,其吸光度变化符合Lambert-Beer定律,标准曲线的回归方程为y=0.0039x-0.0151（x为双波长吸光度差值）,r=0.9972,结果表明,用此方法测定操作简便、回收率高、重现性好,且相对误差小;所测10种饲料原料的木聚糖含量在2.69%-16.78%之间。The D-xylose as the standard material,the orcinol·HCl as the display reagent,we had established a method to mensurate the content of the xylan in the feed raw material by a dual-wave length spectrophotometry.In the response solution the D-xylose density is 0～100 μg/mL,the change of the absorbency was accordant to Lambert-Beer law,the standard curve regression equation was y=0.0039x-0.0151（x was the double wave length extinction interpolation）,r= 0.9972.The results indicated that this method was simple in operation,high in returns-ratio,well in the reproduction quality,small in the relative error.10 kind of feed raw materials were mensurated in this method,the results showed that the content of the xylan was 2.69%～16.78%.

关 键 词：木聚糖 双波长差值法 饲料原料 

分 类 号：S816[农业科学—饲料科学]