加工番茄遗传转化再生体系的建立  

Processing Tomato Plant Regeneration in Genetic Transformation

作　　者：张录霞[1] 郝青南[1] 马超[1] 段小瑜[1] 牛建新[1] 马兵钢[1] 

机构地区：[1]石河子大学农学院园艺系,新疆832000

出　　处：《西北农业学报》2008年第3期236-241,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(30460081);新疆自治区高校科研计划(XJEDU2005S15)项目

摘　　要：加工番茄种子出芽后7-8 d,子叶剪成约0.2-0.4 cm,0.3-0.5 cm的小块,以MS＋B5为基本培养基,附加IAA0.2 mg/L分别与0.5,1.0,1.5,2.0,2.5 mg/L的6-BA/ZT/TDZ组合;6-BA2.0 mg/L分别与0.0,0.05,0.1,0.2,0.5,1.0 mg/L的IAA/IBA/NAA组合。经诱芽比较,在不同浓度6-BA/ZT/TDZ与0.2mg/LIAA组合中,出芽率最高的培养基为MS＋ZT1.0 mg/L＋IAA0.2 mg/L和MS＋TDZ2.0 mg/L＋IAA0.2 mg/L。在不同浓度的IAA/IBA/NAA与6-BA2.0 mg/L组合中,IAA明显优于NAA和IBA,筛选出MS＋2.0 mg/L6-BA＋1.0 mg/LIAA为最佳生芽培养基。以1/2MS添加NAA//BA0.0,0.1,0.2,0.5,1.0 mg/L进行生根比较,再生芽在MS＋0.5 mg/L IBA生根培养基上生根最好,并发育成完整的小植株。When tomato seedlings grew 7-8 days after germination cotyledons were cut into 0.2-0.4,0.3-0.5 cm discs as explants.MS＋B5 was used as basic medium,and IAA0.2 mg/L combined with different concentrations of 6-BA/ZT/TDZ on bud induction,6-BA2.0 mg/L combined with different concentrations of IAA /IBA/NAA on bud induction.The results showed that the best shoot induction medium for cotyledon explants was MS＋ZT1.0 mg/L ＋IAA0.2 mg/L and MS＋TDZ2.0 mg/L＋IAA0.2 mg/L in different combinations of IAA0.2 mg/L and 6-BA/ZT/TDZ.Among different combinations of 6-BA2.0 mg/L and IAA/IBA/NAA,IAA was bester than that of IAA and IBA on bud induction.The best medium on bud induction was MS ＋ 6-BA 2.0 mg/ L ＋ IAA 1.0 mg/ L.The root could be induced after the regenerated buds were planted in M S＋ IBA 0.5 mg/L one week later.

关 键 词：加工番茄 遗传转化 再生体系 子叶 

分 类 号：S641.2[农业科学—蔬菜学]