龙葵碱对HepG_2细胞磷脂酰丝氨酸及断裂DNA研究  

Study on PS and cracking DNA in HepG_2 cells induced by solanine

作　　者：季宇彬[1] 徐丽丽[1] 高世勇 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站

出　　处：《哈尔滨商业大学学报（自然科学版）》2008年第3期268-273,共6页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金项目(30400591);黑龙江省自然科学基金项目(D2004-13;D200505);哈尔滨市青年科学基金项目(2004AFQXJ035)

摘　　要：研究龙葵碱对肝癌细胞HepG2的诱导凋亡作用.采用倒置显微镜观察形态学变化,Annexin V-FITC联合PI双染法和Tunel法检测龙葵碱诱导人肝癌HepG2细胞凋亡作用.结果发现龙葵碱作用24 h后,贴壁细胞数量减少、体积缩小,变圆等明显凋亡形态;在激光共聚焦显微镜下观察到AnnexinV-FITC阳性信号为绿色荧光,PI为红色荧光;Tunel阳性信号为绿色荧光.表明龙葵碱通过诱导HepG2细胞凋亡达到抗肿瘤的作用.To investigate the apoptosis effects of solanine in HepG2 cell. The morphology of cells is observed under transmission electron microscopy. Apoptosis of solanine is assessed with Annexin V - FITC/PI and Tunel. The result is that with the inverted microscope, after being treated with drug-solanine , cells grew against the wall and peeled off gradually, which showed the characters of cells apoptosis. With the laser confocal microscopic, the positive signal of Annexin V - FITC is green, and PI is red ; the positive signal of tunnel is green. Inhibition on tumor of solanine can be accomplished through inducing the apoptosis in HepG2 cells.

关 键 词：龙葵碱 细胞凋亡 ANNEXIN V-FITC联合PI双染 TUNEL 

分 类 号：R285[医药卫生—中药学]