非小细胞肺癌中p14ARF和mtp53蛋白表达与临床病理参数的关系  

Relationship between the expession of p14ARF,mtp53 protein and clinical pathological parameters in non-small cell lung cancer

作　　者：杨廷桐[1] 武俊芳[1] 李秀杰[2] 范文艳[1] 汪艳丽[1] 

机构地区：[1]新乡医学院形态学实验室,河南新乡453003 [2]新乡医学院诊断学实验室,河南新乡453003

出　　处：《新乡医学院学报》2008年第4期336-339,共4页Journal of Xinxiang Medical University

基　　金：河南省教育厅资助项目(编号:2006310024)

摘　　要：目的探讨p14ARF和mtp53蛋白在非小细胞肺癌(NSCLC)中的表达与临床病理参数的关系。方法采用免疫组织化学S-P法检测15例正常肺组织、18例癌旁组织和98例NSCLC组织标本中p14ARF和mtp53的表达。结果(1)在正常肺组织、癌旁组织和NSCLC中p14ARF蛋白的阳性表达率分别为93.3%、83.3%、55.1%,mtp53蛋白的阳性表达率分别为6.7%、44.4%、79.6%;p14ARF蛋白和mtp53蛋白在NSCLC中的表达与正常肺组织和癌旁组织比较均有显著性差异(P<0.01);p14ARF蛋白表达与临床病理分期、有无淋巴结转移、有无脉管浸润无关(P>0.05),而与病理分化程度有关(P<0.01);mtp53蛋白表达与临床病理分期、病理分化程度(P<0.01)及有无脉管浸润(P<0.05)有关,而与有无淋巴结转移无关(P>0.05);(2)在NSCLC中,pl4ARF与mtp53蛋白共表达为36.7%,一致性为38.8%,二者表达呈负相关(r=-0.3553,P<0.001)。结论p14ARF表达下调及mtp53表达上调在NSCLC的发生、发展中发挥着一定的作用;联合检测p14ARF和mtp53有助于判断NSCLC的分级和转移情况。Objective To investigate the relationship betweent the expession of p14ARF,mtp53 protein and clinical pathological parameters in non-small cell lung cancer（NSCLC）. Methods The expression of p14ARF and mtp53 proteins in 15 cases of normal lung,18 cases of tissue adjacent to the cancer and 98 cases of NSCLC were detected with the S-P immunohistochemical method. Results （ 1 ） In normal lung, tissues adjacent to the cancer and NSCLC tissues, the positive expression of p14ARF protein was 93.3 %, 83.3 % and 55.8% respectively, and the mtp53 protein expression was 6.7% ,44.4% and 79.6% respectively. The positive expression both of p14ARF and mtp53 protein in NSCLC tissues were significantly different from that in nonmal lung tissues and tissues adjacent to the cancer （P 〈0. 01 ）. The expression of p14ARF protein was correlated with pathological degree（P 〈 0. 01 ）, but it was uncorrelated with pathological staging, lymphaden metabasis and vessel invasion（ P 〉 0. 05 ）. The expression of mtp53 protein was correlated with clinical pathology staging,pathological degree and vessel invasion（ P 〈0.05 or P 〈 0. 01 ）, but it was uncorrelated with lymphaden metabasis （P 〉 0. 05 ）. （2） The coexpression of p14ARF and mtp53 was 36.7% ,their consistency was 38.8% ,but the expression of p14ARF protein were negatively correlated with the expression of mtp53 protein（ r = -0. 3553 ,P 〈 0. 001 ）. Conclusion The results suggest that the decrease of p14ARF and the increase of mtp53 protein may play an important role in the genesis and development of NSCLC. The p14ARF and mtp53 was negative correlation. Binded detections of p14ARF, mtp53 protein contribute to diagnosis of pathology grade and metastasis of NSCLC.

关 键 词：非小细胞肺癌 P14ARF蛋白 mtp53蛋白 临床病理参数 

分 类 号：R734.2[医药卫生—肿瘤]