两种色型黄粉虫杂交后代过氧化物酶同工酶及超氧化物歧化酶活性的比较  

Comparison of the Peroxidase Isozymes and SOD Activities of the Hybrids of Two Colour Types Tenebrio Molitor

作　　者：吉志新[1] 王长青[2] 史凤玉[1] 刘艳丽 

机构地区：[1]河北科技师范学院农学系,河北秦皇岛066600 [2]河北科技师范学院图书馆,河北秦皇岛066600 [3]秦皇岛市北戴河区旅游局

出　　处：《河北科技师范学院学报》2008年第2期18-22,39,共6页Journal of Hebei Normal University of Science & Technology

基　　金：秦皇岛市科委资助项目;河北省教委资助项目(项目编号:2003308)部分内容

摘　　要：采用聚丙烯酰胺凝胶电泳法测定了黄粉虫两种色型杂交后代的三种虫态（老熟幼虫、蛹和成虫）的过氧化物酶同工酶，用NBT光化还原法测定了不同虫态的SOD活性。结果表明：黄粉虫幼虫-蛹-成虫过氧化物酶同工酶的酶活性均呈现强-弱-强的变化规律。杂交后代的各虫态，除雌蛹外其SOD活性均高于其自交后代的SOD活性；以黄色型黄粉虫为母本的雌、雄成虫和雄蛹的SOD活性较高，而以黑色型黄粉虫为母本的雌蛹和幼虫的SOD活性较高。To analyze the peroxidase isozymes and SOD activities of the hybrids of two colour types Tenebrio molitor in different developmental stages（ matured larvae, pupas and adult）, polyacrylamide gel electrophoresis and NBT photoreduction method were used in this paper. The results showed that the activities of peroxidase isozymes were the highest in the adult stage and the larvae stage. Except for female pupas, SOD activities of progenies of the hybrids in different developmental stages were significantly higher than that of progenies of self-cross. Using yellow-type T. molitor as female parent,SOD activities of progenies of the hybrids were high in male adult,female adult and male pupas. However,SOD activities of progenies of the hybrids were high in female pupas and larvae stage when black-type T. molitor were used as female parent.

关 键 词：黄粉虫 过氧化物酶 电泳 超氧化物歧化酶 

分 类 号：Q969.498.206[生物学—昆虫学]