雷公藤内脂醇对人卵巢癌耐顺铂细胞株超微结构的影响  

Effect of Triptolide on the Ultrastructure of COC_1/DDP

作　　者：罗丽萍[1] 谭布珍[1] 袁铿[2] 黄艳琴[2] 胡银英[2] 袁芳[2] 虞功清[3] 

机构地区：[1]南昌大学第二附属医院妇产科 [2]江西省医学科学研究所肿瘤室,南昌330006 [3]江西省医学科学研究所电子显微镜室,南昌330006

出　　处：《实用临床医学（江西）》2008年第6期5-7,共3页Practical Clinical Medicine

摘　　要：目的探讨雷公藤内脂醇对体外培养的人卵巢上皮性癌耐顺铂细胞株(COC1/DDP)超微结构的影响。方法空白对照组加入9mLCOC1/DDP专用细胞培养液培养,雷公藤内脂醇作用组加入9mL含50μg/L雷公藤内脂醇溶液培养,2组作用48h后收集细胞,通过透射电子显微镜观察COC1/DDP的超微结构。结果透射电子显微镜下观察可见空白对照组中COC1/DDP细胞膜表面有丰富的微绒毛,细胞核大而明显,染色质丰富;雷公藤内脂醇作用组中COC1/DDP细胞表面微绒毛消失,细胞核固缩、染色质凝集成新月状,有凋亡小体形成。结论50μg/L雷公藤内脂醇可诱导COC1/DDP呈凋亡改变。Objective To investigate the effect of triptolide on the ultrastructure of COC1/DDP in vitro. Methods In blank contrast group, COC1/DDP was cultivated with 9 mL COC1/DDP Culture medium;and in the triptolide experimental group,COC1/DDP was cultivated with 9 mL triptolide solution in concentration of 50 μg/L. After the treatment for 48 h, the cells were collect- ed and observed for the COC1/DDP ultrastructure by transmission electron microscopesh. Results In blank contrast group,there were rich microvilli on COC1/DDP cell surface and obvious nucleolus in the cell and abundant ricosomes in cytoplasm;As in triptolide experimental group,microvillus on the surface of COC1/DDP cells disappeared,cell-nucleus was condensed, and chromosomal was agglutinated into crescent, apoptosis body had formed. Conclusion The triptolide in concentration of 50 μg/L may induce apoptosis of the COC1/DDP.

关 键 词：雷公藤内脂醇 人卵巢癌耐顺铂细胞株 超微结构 

分 类 号：R361.3[医药卫生—病理学] R737.31[医药卫生—基础医学]