NSPc1相互作用蛋白的筛查及其与组蛋白乙酰化酶HBO1相互作用的验证  

Screening and verification of the interactive proteins for nervous system polycomb 1

作　　者：龚燕华[1] 石磊[1] 吴旭东[1] 强伯勤[1] 彭小忠[1] 袁建刚[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2008年第6期529-534,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(30600166;30721063);"863"课题(2006AA02Z137)

摘　　要：目的寻找多梳家族(polycomb group)蛋白NSPc1的体内可能的相互作用蛋白。方法通过酵母双杂交实验筛选与NSPc1相互作用的蛋白。使用Pull-down实验,Co-IP实验以及细胞内荧光共定位实验进一步证实双杂交中发现的相互作用。结果分别以NSPc1蛋白的全长、N端和C端作为诱饵筛查人3月胎脑cDNA文库,发现N端诱饵可以与组蛋白乙酰化转移酶HBO1相互作用,该相互作用在体内外实验中都得到了验证。结论组蛋白乙酰化酶HBO1是NSPc1的相互作用蛋白之一,该相互作用可能参与NSPc1对靶基因转录的表观抑制活性的发挥。Objective To find out the protein complex members related to the nervous system polycomb 1 （ NSPcl ） in vivo. Methods Use the yeast double hybrid system to screen the potential interactive proteins of NSPcl, and verify the interaction by GST pull-down, co-immunoprecipitation and subcellular co-localization assays. Results The full length, N-terminus and C-terminus of NSPcl were used as the ＂baits＂ to screen the 3-month fetal brain cDNA library in yeast. Some proteins, including HBO1 （ Histone Acetyltransferase Binding to ORC-1 ）, were found to be interactive proteins of NSPcl, then the interaction between HBO1 and NSPcl was verified both in vitro and in vivo. Conclusion The histone acetyltransferase HBO1 is one of the interactive proteins of NSPcl, and the interaction may be involved in the epigenetic repression activity of polycomb proteins.

关 键 词：多梳家族 神经系统多梳蛋白1 酵母双杂交 蛋白质相互作用 组蛋白乙酰化酶 

分 类 号：Q51[生物学—生物化学]