帕金森病模型大鼠中脑黑质磷酸化α-突触核蛋白表达增加  

Increased expression of phosphorylated α-synuclein in the substantia nigra of parkinson's disease rat model

作　　者：隋雷鸣[1] 赵焕英[1] 赵春礼[1] 孙晓红[1] 杨慧[1] 

机构地区：[1]首都医科大学北京神经科学研究所北京神经再生修复研究重点实验室教育部神经变性病学重点实验室,北京100069

出　　处：《基础医学与临床》2008年第6期570-574,共5页Basic and Clinical Medicine

基　　金：973国家重点基础研究发展计划项目(2006CB500706);国家自然科学基金(30670655;7042008);北京市教育委员会科技发展计划项目(KM200610025002);教育部高等学校博士学科点专项科研基金项目(20060025004)

摘　　要：目的观察鱼藤酮对大鼠中脑黑质磷酸化α-突触核蛋白(α-SYN)、酪氨酸羟化酶(TH)表达的影响。方法利用脑立体定位技术注射微量鱼藤酮,建立急性损伤帕金森病(PD)模型。采用动物行为轨迹分析软件计算各时间点30min内运动距离和平均运动速度。免疫组织化学检测黑质(SN)部位TH、磷酸化α-SYN的表达,并统计TH和磷酸化α-SYN阳性神经元数目。结果PD组动物各时间点30min内运动距离、平均运动速度低于对照组(P<0.05,0.01)。同对照组相比,损伤侧SN部位TH阳性神经元数目明显减少(P<0.01),磷酸化α-SYN阳性神经元数目明显增加(P<0.01)。结论PD大鼠中脑黑质磷酸化α-SYN表达增加及TH的表达明显下降,并出现运动障碍。Objective To observe the effect of rotenone on phosphorylated α-SYNuclein and TH expression in rat SN of PD. Methods Rats were stereotactically injected with rotenone. Movement distance and mean speed within 30-minutes were calculated with Ethovison software. TH and phosphorylated α-SYN expression of the lesioned SN were demonstrated with immunohistochemical staining. The number of TH-pesitive neurons and phosphorylated α-SYN-pesitive neurons in the lesioned SN were measured. Results Movement capability and mean speed in the PD group were decreased than those in the control group（P 〈0. 05, 0. 01 ）. Compared to control group, TH-posi- tire neurons counts in the lesioned SN in the PD group was reduced（ P 〈 0. 01 ）. The numbers of α-SYN-pesitive neurons were significantly more in the PD group than in the control group （ P 〈 0. 01 ）. Conclusion Injection with rotenone could induce PD symptom, TH-pesitive neurons decrease and phosphorylated α-SYN increase.

关 键 词：磷酸化α-synuclein 鱼藤酮 酪氨酸羟化酶 帕金森病 

分 类 号：R742.5[医药卫生—神经病学与精神病学]