新疆杏树苹果锈果类病毒的检测与全序列分析  

Detection and Sequencing of Apple scar skin viroid from Apricot in Xinjiang

作　　者：赵英[1] 牛建新[1] 

机构地区：[1]石河子大学农学院园艺系,新疆石河子832003

出　　处：《园艺学报》2008年第6期805-810,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30360066);国家科技攻关计划引导项目(2003BA546C);兵团科委项目(NKB02SDXNK01SW)

摘　　要：在以往研究苹果锈果类病毒（ASSVd）的基础上，以斑点杂交检测ASSVd呈阳性的杏树为试验材料，利用RT-PCR技术扩增出苹果锈果类病毒全长特异片段，通过回收克隆测序，发现杏树苹果锈果类病毒基因组长为330nt，将克隆测序结果在GenBank中登录，获得10条序列，登录号为EU031487-EU031496。在此基础上，利用原位RT-PCR检测技术，进一步证明杏树叶片中有苹果锈果类病毒存在，主要分布在细胞核中。本研究证实苹果锈果类病毒也能侵染杏树。Present study was conducted based on the previous research of Apple scar skin viroid（ASSVd）for apple and pear,used material of apricot was those that showed positive signal of blot hybridization detection.Full-length special fragment of ASSVd for apricot was amplicated using RT-PCR protocol,then was recycled,cloned and sequenced.It was demonstrated that the full-length genome of ASSVd of apricot is 330 nt long（GenBank numbered with EU031487 to EU031496）.The exist of ASSVd in apricots was reconfirmed by the results of in situ RT-PCR,and ASSVd mainly located in nucleolus of mesophyll cell.The fact of infecting capability of apricot enlarged the host ranges of ASSVd.

关 键 词：杏树 病毒 苹果锈果类病毒 斑点杂交 RT-PCR 原位RT-PCR 全序列分析 

分 类 号：S662.2[农业科学—果树学]