EB病毒转化B淋巴细胞建立的永生细胞株长期传代遗传稳定性的研究  

Study on the genetic stability of immortalized cell line of lymphocyte cell transformed by EB virus after long subculture process

作　　者：李彦涵[1] 黄小琴[1] 林克勤[1] 陶玉芬[1] 易文[1] 姚宇峰[1] 史磊[1] 褚嘉祐[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所遗传室,昆明650118

出　　处：《中华医学遗传学杂志》2008年第3期276-279,共4页Chinese Journal of Medical Genetics

基　　金：美国中华医学基金会资助项目（CMB04-805）;志谢 云南省第一省人民医院遗传诊断中心朱宝生主任等对染色体实验给予支持,特致谢意.

摘　　要：目的研究EB病毒转化外周血B淋巴细胞建成的永生细胞株在传代过程中遗传特性是否发生改变。方法对10株永生细胞株进行30代的长期传代培养，采用染色体显带、微卫星DNA及端粒酶活性检测技术，观察不同传代次数的永生细胞株二倍性、染色体核型、微卫星DNA及端粒酶活性的改变。结果永生细胞株在30代以内，在染色体二倍性、核型、微卫星DNA方面保持稳定，未检测出端粒酶活性。结论经EB病毒转化的永生细胞株在有限代次内遗传特征是稳定的。Objective To study the genetic stability of an immortalized cell line transformed by Epstein-Barr virus （EBV） after long subculture process. Methods In the present study, the genetic stability including chromosome diploidy, karyotypes and micmsatellite DNA were evaluated with chromosome banding techniques and micmsatellite DNA detection. The telomerase activity of the immortalized cell line was detected by using the telomerase assay kit. Results From passage 1 to 30, there were no change of the diploidy, karyotypes of chromosome and micmsatellite DNA, and the telomerase activity is negative. Conclusion This study indicates that the immortalized cell line remains stable genetically within limited passages.

关 键 词：永生细胞株 染色体二倍性 微卫星DNA 端粒酶活性 遗传稳定性 

分 类 号：R686[医药卫生—骨科学]