肾癌相关基因CXorf36的克隆及亚定位研究  

Cloning and Sublocalization Analysis of a Novel Renal Cancer-associated Gene CXorf36

作　　者：卓德祥[1] 徐洪亮[1] 赵磊[1] 李载权[1] 毛泽斌[1] 

机构地区：[1]北京大学医学部生物化学与分子生物学系,北京100083

出　　处：《中国生物化学与分子生物学报》2008年第6期531-536,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助项目(No.30471914)~~

摘　　要：应用生物信息学分析,筛选获得1个肾癌组织中高表达,而在癌旁正常组织中低表达的新基因CXorf36(chromosome X open reading frame 36),并用RT-PCR方法,在组织和细胞系中加以验证.与肾癌组织相比,3种肾癌细胞系中该基因mRNA的表达丰度很低.为观察CXorf36基因表达产物在哺乳动物细胞内的亚定位,构建了pEGFP N1-CXorf36融合表达载体,转染786-O细胞,其融合蛋白均匀分布于细胞浆.氨基酸序列比对分析显示,CXorf36蛋白与小鼠来源的猜测蛋白LOC75905具有79%的同源性,而小鼠LOC75905蛋白可能是一种水解酶.因此,推测CXorf36蛋白是通过其水解酶活性,在肿瘤的发生、生长或侵袭过程中发挥重要作用.进一步关于CXorf36基因功能的研究,如其在肿瘤组织中的定位、对细胞生物学功能的影响,及其寻找相互作用分子的实验等仍在进行中.To study CXorf36（chromosome X open reading frame 36）,a new renal cell carcinoma-associated gene was identified in our previous work screening for tumor-associated antigen.RT-PCR was used to detect expression profile in renal cell carcinoma tissue and cell lines.The results showed that CXorf36 mRNA was expressed much higher in renal cell carcinoma tissue than normal renal tissue,whereas the CXorf36 mRNA levels in three kinds of renal cell carcinoma cell lines were much lower compared to cancer tissue.To investigate the sublocalization of CXorf36 protein in mammalian cells,the pEGFP N1-CXorf36 fusion expression vector was constructed and transfected into the human 786-O cell line.The fusion protein was localized in the cytoplasm.Amino acid sequence alignment indicated that CXorf36 shared 79% identity with mouse hypothetical protein,LOC75905,which was a putative hydrolase.Hence we hypothesized that CXorf36 protein might play a crucial role in tumorigenesis,tumor growth or tumor invasion through its hydrolase activity.More functional analyses about CXorf36 such as its localization in tumor tissue,effects in cell biology,yeast two hybrid assay searching for potential partner are under way.

关 键 词：肾癌 基因 水解酶 

分 类 号：R73[医药卫生—肿瘤]