米曲霉β-呋喃果糖苷酶基因克隆及生物信息学分析  

Cloning and Bioinformatic Analysis of β-fructofuranosidase Gene from Aspergillus oryzae

作　　者：唐江涛[1] 覃益民[1] 杨梅[1] 姚评佳[2] 魏远安[3] 唐尹萍[4] 

机构地区：[1]广西大学化学化工学院,广西南宁530004 [2]广西大学生命科学与技术学院,广西南宁530004 [3]广西大学亚热带生物资源保护利用重点实验室,广西南宁530004 [4]湖北中医学院药学院,湖北武汉430065

出　　处：《食品科学》2008年第6期208-211,共4页Food Science

基　　金：广西高校博士点建设资金项目

摘　　要：β-呋喃果糖苷酶水解蔗糖和低聚果糖,酶法生产高纯度低聚果糖,必须抑制或消除β-呋喃果糖苷酶的水解活性。本研究以工业生产低聚果糖的米曲霉菌株GX0015为研究材料,采用RT-PCR技术,克隆获得β-呋喃果糖苷酶基因(GenBank登录号:EU130944)。利用生物信息学手段对β-呋喃果糖苷酶基因进行分析得知:该酶为456个氨基酸组成的亲水性膜外蛋白;功能域分析结果显示:该酶从第52个至第456个氨基酸残基之间序列属于糖苷酶32家族特征序列,并具有糖苷酶32家族酶活性中心的(N/G)DP(C/N)G和RDP保守序列;米曲霉β-呋喃果糖苷酶在进化树上的位置处于酵母菌的转化酶和细菌的六磷酸蔗糖水解酶之间。β -fructofuranosidase （FRU） hydrolyzes sucrose and fructooligosaccharide （FOS）. Therefore, the FRU enzymatic activity should be inhibited or eliminated for the production of FOS enzymatically. In this study, a β -fructofuranosidase gene was cloned with RT-PCR from Aspergillus oryzae GX0015 which is a key strain for FOS production industrially. This gene was sequenced and submitted to GenBank （accession number： EUl30944）. The sequence was analyzed bioinformatically. The results showed that FRU composing of 456 amino acids is a hydrophilic protein without transmembrane helices. The glycoside hydrolysis family 32 domain is present in the 52th to 456th amino acid of FRU and （N/G）DP（C/N）G and RDP conserved sequences are located in the active center of FRU. The phylogenetic tree shows that FRU ofAspergillus oryzae is between yeast invertase and bacterial sucrose-6-phosphate hydrolase.

关 键 词：Β-呋喃果糖苷酶 克隆 生物信息学分析 

分 类 号：Q785[生物学—分子生物学] Q811.4