尿液脱落细胞CD_(44)基因拼接变异体对膀胱癌的诊断价值  

Diagnostic value of CD 44 splice variants in urine exfoliated cells of bladder cancer

作　　者：吴忠[1,2,3] 张元芳[1,2,3] 刘定干 李载平[1,2,3] 贺斌 

机构地区：[1]上海医科大学华山医院泌尿外科 [2]中国科学院上海生物化学研究所 [3]上海医科大学肝癌研究所

出　　处：《中华外科杂志》1997年第9期533-535,共3页Chinese Journal of Surgery

摘　　要：作者应用逆转录－聚合酶链反应和Ｓｏｕｔｈｅｒｎ杂交技术对２０例膀胱癌和２０例非癌对照组尿液脱落细胞ＣＤ４４基因拼接变异体进行检测，并与尿细胞学检查结果进行比较。结果显示：９０％的膀胱癌患者尿液脱落细胞均检测到ＣＤ４４基因拼接变异体的过量表达，而２０例非癌对照标本均未检测到这种异常。本技术诊断膀胱癌的敏感性为９０％，较尿细胞学检查的６５％明显提高。提示尿液脱落细胞ＣＤ４４基因拼接变异体是一种很有前途的用于膀胱癌早期无创伤性诊断的新的分子标记物。To study the early and non invasive diagnostic value of CD 44 splice variants in urine exfoliated cells of bladder cancer.We used reverse transcriptionpolymerase chain resction (RT PCR) and southern blot hybridization to detect CD 44 splice variants in exfoliated cells in 40 urine samples (20 bladder cancers, 20 non neoplastic controls),and compared with the results of urine cytology on the same set of samples.90%(18/20) of the urine samples of bladder cancer showed overexpression of CD 44 splice variants while none of the 20 controls did so. This method not only has a sensitivity of 90%( 18/20) which was much higher than that of 65%(13/20) by using urine cytology, but also is non invasive and comfortable. The results suggest that CD 44 splice variants in exfoliated cells in urine samples are a new tumor marker for early and non invasive diagnosis of bladder cancer.

关 键 词：膀胱肿瘤 尿液 诊断 CD44基因 

分 类 号：R737.140.4[医药卫生—肿瘤] R730.43[医药卫生—临床医学]