遗传稳定型六六六、甲基对硫磷降解基因工程菌的构建及特性研究  

Construction of a Stable Genetically Engineered Microorganism for Degrading HCH & Methyl Parathion and Its Characteristics

作　　者：陆鹏[1] 洪源范[1] 洪青[1] 蒋新[2] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,南京210095 [2]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京210008

出　　处：《环境科学》2008年第7期1973-1976,共4页Environmental Science

基　　金：国家自然科学基金项目(30400013);江苏省科技厅项目(BG2005322);江苏省教育厅项目(JHZD06-2);中国博士后科学基金项目(2005037747);江苏省博士后科研计划项目

摘　　要：通过转座子介导同源重组的方法,将甲基对硫磷水解酶基因mpd导入到六六六降解菌BHC-A的染色体上,构建了能同时降解六六六和甲基对硫磷的基因工程菌BHC-A-mpd.对其生长特性和降解特性的研究表明,工程菌在LB培养基中的生长特性与原始菌株没有差别,生长至对数期A600 nm值都可达到2.5;对六六六的降解特性和原始菌株相同,可在10 h内将5 mg/L的六六六完全降解.mpd基因在BHC-A-mpd中稳定表达,BHC-A-mpd可以降解多种有机磷类农药.该基因工程菌的构建为六六六和甲基对硫磷复合污染环境的生物修复奠定了基础.A GEM designated as BHC-A-mpd, capable of simultaneously degrading of methyl parathion（MP） and HCH was successfully constructed by random insertion of a methyl parathion hydrolase gene （mpd） into chromosome of a HCH-degrading strain BHC-A with the mini- Tn-transposon system .The growth and degrading characteristics of BHC-A-mpd was compared with the original strain BHC-A, and the result showed that there was no difference in this two aspects, A600nm of BHC-A-mpd in LB medium could reach 2.5 in logarithmic period, which was the same as that of the original strain BHC-A. BHC-A-mpd showed the same HCH-degrading ability as BHC-A and could degrade 5 mg/L of y-HCH in 10 h. BHC-A-mpd showed high genetica stability and could degrade many kinds of organophosphorus pesticides. All these results indicated that BHC-A- mpd was a promising GEM in bioremediation of MP and HCH co-contaminated environment.

关 键 词：六六六 甲基对硫磷 生物降解 基因工程菌 

分 类 号：X172[环境科学与工程—环境科学]