Detection for Transcriptional Activity of Alternaria Tenuissim Protein Elicitor in Yeast Two-hybrid System  

极细链格孢菌蛋白激发子PeaT1在酵母双杂交系统中转录激活活性的检测（英文）

作　　者：刘延锋[1] 邱德文[1] 曾洪梅[1] 杨秀芬[1] 

机构地区：[1]中国农业科学院植物保护研究所

出　　处：《Agricultural Science & Technology》2008年第1期64-66,共3页农业科学与技术（英文版）

基　　金：Supported by the National“863”Program(2006AA10A210)~~

摘　　要：The peaT1 gene fragment was amplified from pGEM-6p-l-peaT1 by PCR, and recovered target gene was cloned into pLexA vector. After digestion and sequencing, the bait vector pLexA-peaT1 was transformed into yeast strain EGY48 [p8op-lacZ] by PEG/LiAC, and the transcriptional activity of bait vector was detected. The results showed that recombinant bait plasmid pLexA-PEMG1 was constructed, for the two bands of recombinant bait plasmid in agarose gel eleetrophoresis were expected after digesting by restriction endonuclease EcoR I and Xho I. Therefore, the recombinant bait plasmid could be used in yeast two-hybrid system to screen a cDNA library.2000年笔者从植物病原真菌中提取获得一类新型蛋白激发子，它能提高植物自身免疫力，促进植物生长，提高作物产量和产品品质。并在研究该类蛋白的过程中，从极细链格孢菌中分离纯化到1种能诱导植物产生系统抗性、促进植物生长的蛋白，并进一步克隆了编码该蛋白的基因peaTl（Genbank登录号CH445335）。为进一步阐明PeaTl的分子作用机理，拟采用酵母双杂交技术，

关 键 词：PeaT1 Yeast two-hybrid Transcriptional activity 

分 类 号：S188[农业科学—农业基础科学]