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机构地区:[1]辽宁石油化工大学环境与生物工程学院,辽宁抚顺113001 [2]沈阳农业大学生物科学技术学院,沈阳110161
出 处:《玉米科学》2008年第1期83-85,90,共4页Journal of Maize Sciences
基 金:国家重点基础研究发展计划(G1999011707)
摘 要:研究耐低钾玉米自交系在光合特性上的耐低钾机制。玉米自交系种子A(不耐低钾)和B(耐低钾),用3个不同钾离子浓度(5、100、1000μmol/L)进行液体培养,4周时将苗转入培养桶中,连续培养50d。测定其光合速率、荧光效率、PEP羧化酶(PEPase)、ATP酶(ATPase)。结果表明,低钾条件下,自交系B的光合速率、PEPase、ATPase活性显著高于自交系A,荧光效率彼此无显著性差异。因此,低钾条件下,光能转化效率不是光合作用的限制因子,耐低钾自交系通过提高光合作用的暗反应而提高光合作用,从而适应低钾逆境。Arm at studying mechanism of tolerant to K^+ deficiency on the photosynthesis characteristics of maize seedling of B. Two inbred lines of maize seed, A which sensitive to K^+ deficiency and B which tolerant:to K^+ deficiency, were cultured in nutrient solutions of three different K^+ concentration(5, 100, 1 000 μmol/L). After 4 weeks, the maize seedling that had been cultured was transplanted into cuhure barrel and continued to culture for 50 days. Photosynthetic rate and fluorescence efficiency, PEPase and ATPase of leaves were determined. Results showed that under low K^+ concentration, photosynthetic rate, PEPase and ATPase of B are significantly higher than that of A while fluorescence efficiency has no significant difference each other. The results indicated that light energy transformation efficiency is not restricting factor to photosynthesis under low potassium stress, B improves its photosynthesis through increasing dark reaction so that it adapts low potassium stress.
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