蓝舌病毒群特异性RT-PCR检测技术及其应用  

A Reverse Transcription Polymerase Chain Reaction Method for Detecting of Bluetongue Virus Group

作　　者：尹惠琼[1] 张燕霞[2] 孙宇[1] 张兰兰[1] 孙卫华[1] 杨姝[1] 李晓成[2] 章金刚[1] 

机构地区：[1]军事医学科学院野战输血研究所国家生物医学分析中心病毒安全检测实验室,北京100850 [2]中国动物卫生与流行病学中心,青岛266032

出　　处：《中国动物检疫》2008年第7期28-29,共2页China Animal Health Inspection

基　　金：国家高技术研究发展计划(2006AA10Z446)

摘　　要：目的:建立一种适于蓝舌病毒(BTV)群特异性基因检测的RT-PCR方法。方法:根据本实验室设计的一对BTVNS1基因通用型检测引物建立BTV群特异性RT-PCR检测技术;对不同血清型BTV及鹿流行性出血热病毒(EHDV)进行检测,验证其特异性;同时,利用该方法检测血清模拟样品及抗病毒血清样品。结果:所设计的检测方法特异性好,与EHDV无交叉反应,可检测至少17个血清型BTV,并能有效检出不同病毒浓度的模拟样品及不同型的血清样品。结论:建立的RT-PCR方法可用于BTV群的特异性通用检测。Purpose：Constructing a reliable RT-PCR method for universal detection of various bluetongue virus （BTV） serotypes. Methods：A NS1 gene of BTV detection method was developed by using a pair of novel primers designed before. The specificity of the assay was evaluated by detecting various BTV serotypes and Epizootic hemorrhagic disease virus （EHDV） serotypes. The mimic serum specimens and genuine serum samples from vaccinated sheep were tested. Results：It was show that 17 different serotypes of BTV at least were detected specifically, no cross-reactivity with EHDV. The mimic serum specimens and genuine serum samples were detected successfully. Conclusion：The research indicated that the method is specific and universal for detecting BTV group.

关 键 词：蓝舌病毒 BTV RT—PCR 

分 类 号：S852.65[农业科学—基础兽医学] TS207.3[农业科学—兽医学]