间歇好氧硫酸盐废水处理系统微生物区系解析  

Bacterial community of intermittent aeration sulfate wastewater treatment system

作　　者：赵继红[1] 李继香[2] 何淑英[3] 胡宗泰 王英阁 

机构地区：[1]河南工业大学化学化工学院,郑州450001 [2]同济大学污染控制与资源化研究国家重点实验室,上海200092 [3]华东师范大学环境科学系,上海200062 [4]郑州市污水净化有限公司,郑州450001

出　　处：《环境工程学报》2008年第7期916-921,共6页Chinese Journal of Environmental Engineering

基　　金：河南省杰出青年科学基金资助项目(0512001500)

摘　　要：应用PCR-DGGE(polymerase chain reaction-denaturing gradient gel electrophoresis)技术和16S rDNA序列测定对间歇好氧硫酸盐废水处理工艺的微生物群落结构进行了研究。采集味精厂好氧池原始污泥以及实验室内间歇好氧工艺驯化后不同条件下的活性污泥样品,通过基因组DNA的提取、PCR扩增和DGGE分离,初步分析了各污泥样品的微生物群落多样性,结果表明,PCR-DGGE方法可以在一定程度上反映工艺以及操作条件对微生物群落结构的影响。通过DGGE反复分离纯化及割胶回收,DGGE检验为单一条带后进行测序并提交到GenBank数据库比对,结果表明,间歇好氧硫酸盐系统中优势菌株大多数为未培养细菌,来源于不同的污染环境,具有重要污染物降解的生态功能,其中包括与硫酸盐还原菌(Desulfobulbus propionicus)在系统发育上非常接近的菌株。The microbial community in intermittent aeration process was investigated using the PCR-DGGE （polymerase chain reaction-denaturing gradient gel electrophoresis） and 16S rDNA sequencing methods. Different sludge samples were collected from a monosodium glutamate mill and the laboratory, then the DNA extraction, PCR amplification and DGGE methods were employed to study the variety of bacterial community. It is confirmed that the PCR-DGGE method can reflect the influences of the processes and operating conditions on the microbial community. The single patterns were gained by dint of DGGE purification, and they were reclaimed and delivered to the sequences company. The sequences of the dominant bacteria demonstrated that closely identified relatives in GenBank separated from different polluted environment were uncultured strains with important ecological function, one of which was Desulfobulbus propionicus.

关 键 词：间歇好氧 硫酸盐废水 PCR-DGGE 微生物群落结构 

分 类 号：X172[环境科学与工程—环境科学]