IL-21对非霍奇金淋巴瘤细胞凋亡及survivin基因表达的影响  

Effects of interleukin-21 on apoptosis of non-Hodgkin lymphoma's cell and expression of survivin

作　　者：李丽霞[1] 张英[1] 杨志诚[1] 王玉洲[1] 罗伟仁[2] 

机构地区：[1]广东医学院附属医院肿瘤中心,广东湛江524001 [2]广东医学院病理学教研室,广东湛江524023

出　　处：《海南医学院学报》2008年第4期313-315,共3页Journal of Hainan Medical University

摘　　要：目的:探讨IL-21对非霍奇金淋巴瘤细胞的生长和凋亡的影响及其可能机制。方法:通过MTT(噻唑兰比色法)检测观察IL-21对Raji细胞生长的影响;流式细胞仪观察24 h后IL-21对细胞凋亡的影响;RT-PCR(逆转录-聚合酶链式反应)检测IL-21对survivin mRNA的表达水平的影响。结果:IL-21对Raji细胞生长有抑制作用,呈剂量依赖关系(P<0.05);0,10,50,250μg/L组细胞凋亡率分别为0.00%,7.26%,15.12%,22.57%,药物组与对照组比较,差异有统计学意义(P<0.01或<0.05);随着IL-21浓度升高,survivin的表达水平逐步下降(P<0.05)。结论:IL-21可以抑制淋巴瘤细胞的生长和诱导其凋亡,其机制可能与降低survivin的表达有关。Objective： To study the effects of interleukin-21 （IL-21） on the growth and apoptosis of the non-Hodgkin lymphoma＇s cell and mechanism. Methods： MTT was used to investigate proliferation of Raji ceils; flow cytometry （FCM） was used to investigate the effects of IL-21 on apoptosis of Raji ceils in 24 h; the expression of survivin mRNA in Raji ceils treated with different concentration of IL-21 were examined by RT-PCR. Results： The growth of Raji ceils was inhibited by IL-21, which presented dose-effect relation （ P 〈 0.05 ）. The apoptosis rates of ceils corresponding to concentration of 0,10,50,250 μg/L were 0.00% ,7.26%, 15.12%, 22.57% respectively. Compared the indicators between trial group and control group, there were significant differences （P 〈 0.01 or 〈0.05 ）. The expression rates of survivin mRNA decreased following the gradually increased concentra- tions of IL-21 （ P 〈 0.05 ）. Conclusion： IL-21 can inhibit lymphoma＇s ceil growth and induce cell apoptosis, which may relate to decreasing expression of survivin.

关 键 词：淋巴瘤 非霍奇金 细胞死亡 IL-21 SURVIVIN 

分 类 号：R733.1[医药卫生—肿瘤]