硝基芳烃化合物诱发细胞微核的比较研究  

Comparative Study on Micronucleus Induced by Nitro-aromatic Compounds in Different Cells

作　　者：孔志明[1] 王永兴[1] 臧宇[1] 

机构地区：[1]南京大学,南京210093

出　　处：《上海环境科学》1997年第11期42-44,共3页Shanghai Environmental Sciences

摘　　要：利用蚕豆根尖细胞和人外周血淋巴细胞的微核试验对6种硝基芳烃化合物的致突变性进行比较研究,结果表明,邻二硝基苯、间二硝基苯、2,4-二硝基甲苯、2,6-二硝基甲苯、对硝基氯苯和对硝基溴苯均能诱发两种细胞的微核率增加,除了对硝基溴苯外均具有明显的剂量—效应关系。引进标准剂量概念比较5种硝基芳烃化合物的致突变性,结果表明,致突变性强弱依次为2,4-二硝基甲苯>对硝基氯苯>间二硝基苯>2,6-二硝基甲苯>邻二硝基苯。另外,蚕豆根尖细胞微核方法的检测水平高于人外周血淋巴细胞微核方法。The mutagenicity of O-dinitrobenzene, M-dinitrobenzene, 2,6-dinitrotoluene, 2,4-dinitrotoluene, 4-chloronitrobenzene, 4-bromonitrobenzene were analysis by micronucleus test in vicia faba root tip cells and human peripheral blood lymphocytes. M-dinitrobenzene, 2,4-dinitrotoluene, 2,6-dinitrotoluene and O-dinitrobenzene all induced micronucleus formation in vicia faba root tip cells, and the dose-effect relationships were significant. In human peripheral blood lymphocytes all six nitro-aromatic compounds were positive, and dose-effect relationships were significant except 4-bromonitrobenzene. Compared with the defining dose, the mutagenicity of five nitro-aromatic compounds decreased in order of 2,4-dinitrotoluene, 4-chloronitrobenzene, M-dinitrobenzene, 2,6-dinitrotoluene, O-dinitrobenzene; the detected level of micronucleus test in vicia faba root tip cells was higher than that of micronucleus test in human peripheral blood lymphocytes.

关 键 词：微核 淋巴细胞 硝基芳烃化合物 环境生物学 

分 类 号：X174[环境科学与工程—环境科学]