同步荧光法测定功能饮料中维生素B_2和B_6的研究  

Study on determination of vitamin B2 and B6 in functional drink by synchronous fluorometry

作　　者：徐烨[1] 顾鑫荣[1] 张秀娟[1] 李千[1] 陈立华[1] 

机构地区：[1]东北大学理学院,沈阳110004

出　　处：《分析试验室》2008年第7期85-87,共3页Chinese Journal of Analysis Laboratory

摘　　要：研究了测定功能饮料中维生素B2和B6的同步荧光分析法。确定了最佳实验条件：波长差△λ为70nm，扫描速度为150nm／min，入射和出射狭缝宽度均为10nm，测定波长：B2为448nm，B6为323nm。标准曲线在0．01～0．15μg／mL线性良好，维生素B6和B2的平均回收率分别为96．4％～98．5％和98．3％～100．0％。方法可用于功能饮料中维生素B2和B6的测定。A synchronous fluorometric method was described for the determination of vitamin B2 and B6 in functional drink. The determination condition was studied. Delta lambda was 70 nm, scan speed was 150 rim/min, excitation and emission slit widths were both 10 nm, and determination wavelengthes were： B2/448 nm, B6/323 nm. Under the optimum conditions, the calibration curve was linear between 0.01 - 0.15 μg/mL. The average recoveries of vitamin B6 and B2 were 96.4% -98.5% and 98.3% - 100.0%, respectively. It has been applied to the determination of vitamin B2 and B6 with satisfactory results.

关 键 词：同步荧光法 功能饮料 维生素B2 维生素B6 

分 类 号：O557.3[理学—热学与物质分子运动论]