高效液相色谱-电喷雾离子阱质谱联用法测定金银花中绿原酸和咖啡酸  

Simultaneous determination of chlorogenic acid and caffeic acid in Lonicera japonica by HPLC-ESI-ITMSn

作　　者：李永库[1] 欧小辉[2] 王芬[1] 

机构地区：[1]沈阳农业大学理学院,沈阳110161 [2]广西大学糖业工程技术研究中心,南宁530004

出　　处：《分析试验室》2008年第7期94-97,共4页Chinese Journal of Analysis Laboratory

摘　　要：建立了高效液相色谱．电喷雾离子阱质谱法（HPLC-ESI-ITMSn）同时测定了金银花中绿原酸和咖啡酸活性成分的分析方法。样品用体积分数80％乙醇回流提取，用Zorba）（Eclipse XDB-C18柱分离，以体积分数0．5％甲酸的乙腈．甲酸水溶液为梯度流动相，以保留时间和质荷比对分离出的纽分予以定性确证，用峰面积进行定量。绿原酸和咖啡酸的线性范围均为10．0～1000．0μg/L，检出限（以信噪比为3计）均为2．0μg／L。样品的加标平均回收率为92．7％～98．7％，相对标准偏差为1．4％～2．7％。该方法适用于其它复杂体系中绿原酸和咖啡酸的分析。A method for the determination of biological active components Chlorogenic acid and Caffeic acid in Lonicerajaponica has been developed by high performance liquid chromatography-electrospray ion trap tandem mass spectrometry （HPLC-ESI-ITMS）. The samples were extracted circumfluently with 80% ethanol, Chromatographic separation was performed on a Zorbax Eclipse XDB-C18 column using acetonitrile/water as gradient elution solvents conraining 0.5 % formic acid at a flow rate of 0.25 mL/min. The two compounds were identified by retention time and m/z and quantified by peak area. The results demonstrated that the linear ranges were 10.0 - 1000.0, and 10.0 1000.0 μg/L, with detection limits of 2.0 and 2.0 μg/L, for Chlorogenic acid and Caffeic acid, respectively. The recoveries of the analyte at the spiking concentrations of 10.0 μg/L and 400.0 μg/L were 92.7% - 98.2% and 95.6 % - 98.7 %, respectively, with relative standard deviations of 1.9 % - 2.7 % and 1.4 % - 2.1% （ n = 7）. The proposed method is also suitable for rapid determination of these biological active components in complex matrices.

关 键 词：高效液相色谱-电喷雾离子阱质谱法 金银花 绿原酸 咖啡酸 

分 类 号：O557.63[理学—热学与物质分子运动论]