Differential remodeling of a T-cell transcriptome following CD8- versus CD3-induced signaling  

作　　者：S Hussain I Abidi Tao Dong Mai T Vuong Vattipally B Sreenu Sarah L Rowland-Jones Edward J Evans Simon J Davis 

机构地区：[1]Nuffield Department of Clinical Medicine and MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, TheUniversity of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK [2]Present address: Department of Cell Biology,Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA

出　　处：《Cell Research》2008年第6期641-648,共8页细胞研究（英文版）

摘　　要：CD8 engagement with class I major histocompatibility antigens greatly enhances T-cell activation, but it is not clear how this is achieved. We address the question of whether or not the antibody-mediated ligation of CD8 alone induces transcriptional remodeling in a T-cell clone, using serial analysis of gene expression. Even though it fails to induce overt phenotypic changes, we find that CD8 ligation profoundly alters transcription in the T-cell clone, at a scale comparable to that induced by antibody-mediated ligation of CD3. The character of the resulting changes is distinct, however, with the net effect of CD8 ligation being substantially inhibitory. We speculate that ligating CD8 induces weak, T-cell receptor （TCR）-mediated inhibitory signals reminiscent of the effects of TCR antagonists. Our results imply that CD8 ligation alone is incapable of activating the T-cell clone because it fails to fully induce NFAT-dependent transcription.

关 键 词：receptor triggering signaling CD8 CORECEPTOR SAGE expression analysis 

分 类 号：Q2[生物学—细胞生物学]