不同浓度重组人pcDNA4-VEGI基因对角膜新生血管的抑制作用  

Inhibition of corneal neovascularization by different density of pcDNA_4-vascular endothelial growth inhibitor gene

作　　者：王冰[1] 王利华[1] 钟启文 

机构地区：[1]山东大学附属省立医院眼科,山东省济南市250021 [2]福建省武平县医院五官科,364300

出　　处：《眼科新进展》2008年第7期516-519,共4页Recent Advances in Ophthalmology

摘　　要：目的探讨不同浓度的重组人pcDNA4-血管内皮细胞生长抑制因子(vascular endothelia growth inhibitor,VEGI)基因转移对角膜新生血管(corneal neovascularization,CNV)的抑制作用。方法角膜缝线法制作兔角膜新生血管模型,用结膜下注射的方法将阳离子脂质体包裹的VEGIDNA(剂量分别为10μL、20μL、30μL、40μL)重组质粒(pcD-NA4-VEGI)转染入兔角膜,观察其对CNV的抑制作用。结果基因DNA剂量为10μL时VEGI对CNV的抑制作用不明显,20μL和30μL2种剂量时VEGI作用显著,40μL时作用反而降低。结论阳离子脂质体能够成功介导重组人VEGI基因转染入角膜组织并使之成功分泌VEGI蛋白,20μL时即可对CNV起到较强抑制作用。Objective To evaluate the effects of different density of pcDNA4- vasctdar endothelial growth inhibitor （pcDNA4-VEGI）gene on corneal neovascularization （CNV）. Methods New Zealand albino rabbits were sutured by 54） silk on the superior cornea to establish the animal model and transfected by different density of pcDNA4-VEGI gene （10 μL,20 μL,30 μL.40 μL） mediated by effectene^TM lipofectine transfection. The inhibitive effect on CNV were observed. Results Ten μL of DNA was insufficient to inhibit CNV, 20 μL and 30 μL were effective while 40 μL was diseffective. Conclusion pcDNA,-VEGI gene transfection can be effectively used to inhibit CNV,and 20 μL is the best density of gene DNA.

关 键 词：血管内皮细胞生长抑制因子 阳离子脂质体 基因治疗 角膜新生血管 

分 类 号：R772[医药卫生—眼科]