脑缺血时皮层谷氨酸转运体功能及MDA和SOD的变化  

CHANGE OF GLUTAMATE TRANSPORTER FUNCTION,MDA AND SOD IN CEREBRAL ISCHEMIA

作　　者：任晓燕[1] 侯琳[1] 徐珞[1] 曲忠森 张玉敏[1] 葛宝林[1] 

机构地区：[1]青岛医学院

出　　处：《青岛医学院学报》1997年第3期220-221,共2页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金

摘　　要：①目的探讨脑缺血时皮层突触膜颗粒谷氨酸转运体的功能及丙二醛（ＭＤＡ）、超氧化物歧化酶（ＳＯＤ）的变化。②方法采用大鼠三血管结扎模型，应用放射免疫分析法观察了完全性脑缺血１２０ｍｉｎ时脑皮层突触膜颗粒谷氨酸转运体功能的改变，同时测定了ＭＤＡ含量及ＳＯＤ活力变化。③结果大鼠脑缺血时突触膜颗粒谷氨酸转运体功能和ＳＯＤ明显降低，ＭＤＡ明显增高，与对照组比较差异有显著性（ｔ＝２．８４６，４．９６５，５．０７１，Ｐ＜０．０５，０．０１）。④结论脑缺血时皮层谷氨酸转运体功能降低与自由基损伤有关。Objective To investigate the changes of glutamate transporter function, MDA contents and SOD activity in ischemic synaptosomes in rat cerebral cortex. Methods After complete cerebral ischemia for 120 minutes,glutamate transporter function,MDA and SOD were studied by means of radioimmunoassy and spectrophotometry. Results The glutamate transporter function and SOD activity were significantly decreased( t=4.965,5.071,P <0.01).Contents of MDA were increased( t=2.846,P <0.05). Conclusion Glutamate transporter function and free radical participated in ischemic brain damage.

关 键 词：谷氨酸转运体 脂质过氧化物 脑缺血 MDA SOD 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]