阿霉素诱导S-180瘤细胞凋亡核型分析  

NUCLEUS PATTERN ANALYSIS OF ADRIAMYCIN INDUCED APOPTOSIS IN PARENTAL S 180 CELLS

作　　者：李陆英[1,2,3] 王晓燕[1,2,3] 韩复生[1,2,3] 郭莹 蓝青 

机构地区：[1]青岛医学院生物化学教研室 [2]中国中医研究院针灸所 [3]北京市肿瘤研究所

出　　处：《青岛医学院学报》1997年第2期103-104,共2页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省卫生厅科研基金

摘　　要：①目的探讨阿霉素诱导Ｓ－１８０瘤细胞凋亡过程中细胞核型的变化。②方法用低剂量（３．２５μｇ）阿霉素处理经Ｓ－１８０瘤细胞腹腔接种的ＢＡＬＡ／Ｃ鼠，０～７２ｈ分别取腹水进行细胞核型检测。③结果用阿霉素处理２４ｈ时，细胞变圆，胞浆浓缩；４８ｈ时，染色质密集度增高；７２ｈ时，核仁裂解，核膜内陷，形成内涵物不外溢的凋亡小体。④结论阿霉素是良好的细胞凋亡诱导剂，凋亡期间细胞外形及染色质凝聚逐渐改变，直至形成凋亡小体。Objective To observe the change of nucleus pattern of adriamycin induced apoptosis in parental S 180 cells. Methods Low dosage of adriamycin(3.25ug) was injected into the abdominal cavity of BALA/L mice which were previously inoculated with parental S 180 cells .Nucleus patterns of parental S 180 cells were analysed 0￣72 h after injection of adriamycin. Results The changes of parental S 180 cells were progressive after 24, 48h and 72h of adriamycin injection. Cells turned round, cytoplasm condensed, chromatin density increased, nucleoi splitted, membrane infolded and apoptosis bodies formed. Conclusion Adriamycin is a good apoptosis inducer for S 180 cell line. The process towards apoptosis was gradual until the apoptosis bodyformed.

关 键 词：阿霉素 细胞凋亡 核型 致变物 S-180瘤 

分 类 号：R73－361[医药卫生—肿瘤]