培养时间对黑木耳酯酶同工酶谱的影响  

Effect of Culture Time on the Zymogram of Esterase Isozyme of Auricularia auricula

作　　者：张丕奇[1] 韩增华[1] 戴肖东[1] 马庆芳[1] 孔祥辉[1] 张介弛[1] 

机构地区：[1]黑龙江省科学院微生物研究所,哈尔滨150010

出　　处：《菌物研究》2008年第2期106-109,118,共5页Journal of Fungal Research

基　　金：黑龙江省科学院基金项目(01BFCAHZH10)

摘　　要：为准确地将酯酶同工酶技术应用于食用菌菌株鉴别和遗传育种研究以及进行快速的菌种区分、鉴定,本试验对10个黑木耳菌株不同培养时期的胞内酯酶(EST)同工酶谱进行了研究,并对菌株间亲和性试验结果进行分析。结果表明:同一培养时期的各菌株间同工酶谱存在明显差异,不同培养时期同一菌株同工酶谱也存在一定的差异。培养20d诱导的同工酶谱可有效区分、鉴定各菌株。聚类分析结果表明:培养20d的酶谱聚类分析结果与亲和性试验结果相一致。Compatibility and esterase isozyme zymogram of 10 strains in Auricularia auricula were studied. The results showed that there existed different zymogram polymorphisms during the same culture time of 10 strains. The same strain had different zymogram polymorphisms in different culture time. The esterase isozyme zymogram of strains in 20-day culture can be used for identification of strains. Results of cluster analysis of the esterase isozyme zymogram were consistent with those of the compatibility experiment.

关 键 词：黑木耳 培养时间 酯酶同工酶 聚类分析 亲和性 

分 类 号：Q55[生物学—生物化学] S646.6[农业科学—蔬菜学]