不同微生物等级控制Wistar大鼠部分生物学特性比较  

Comparison on some biologic characteristics of Wistar rats housed in different microbial environment

作　　者：郭雄明[1] 刘亚千[1] 霍永强[2] 李春海[1] 谢忠忱[1] 陈华[1] 

机构地区：[1]解放军总医院医学实验动物中心,北京100853 [2]武警北京总队第二医院,北京100037

出　　处：《军医进修学院学报》2008年第3期173-175,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的:了解不同微生物控制程度(屏障环境和普通环境)对Wistar大鼠的部分生物学特性的影响。方法:40只SPF级Wistar大鼠分别饲养于屏障环境和普通环境内,每周测量体重,35 d后,测定15项血液学指标、14项血液生化指标和9个脏器的相对重量。结果:屏障环境内的Wistar大鼠体重增长明显快于饲养于普通环境的大鼠,普通环境饲养大鼠的血液RBC、WBC、PCT、PLT、GOT、GGT、GPT、CK、TB值高于屏障环境大鼠(P(0.05),而CHO、LDL-C和HDL-C值低于屏障环境大鼠(P(0.05),各脏器相对重量除肾外未见明显差异;结论:饲养于屏障环境和普通环境的Wistar大鼠在体重增长、血液学和血清生化指标等生物学特性存在显著差异。Objective： To investigate the effect of different microbial environment （open system and barrier system） on some biologic characteristics of Wistar rats. Methods ： 40 SPF Wistar rats were assigned into two groups respectively. Group 1 was housed in barrier system, while group 2 was kept in open system. The body weight of rats were measured weekly. After having been kept in the experimental environment for 35 days, 14 hematology and 15 serum chemistry parameters were analyzed and 9 organ weights were measured in rats. Results： Rats housed in barrier systems grew faster than that in open systems （P 〈0.05 ）. The values of RBC, WBC, PCT, PLT, GOT, GGT, GPT, CK, TB of rats kept in barrier system were lower than that in open system（P 〈 0.05 ） , while the values of CHO, LDL-C, HDL-C of rats in barrier system were higher than that in open system （P 〈 0.05 ）. There was no significant difference of relative organ weights between the two groups. Conclusion ： There are some differences of biological characteristics of animals kept in barrier system and open system.

关 键 词：环境设计 环境微生物学 SPF动物 

分 类 号：R446.11[医药卫生—诊断学]