等电聚焦与凝胶电泳在多发性硬化寡克隆带检测中的比较研究  

Comparative study between isoelectric focusing and agarose gel elephoresis in oligoclonal band of multiple sclerosis

作　　者：邹文蓉[1] 张会英[1] 孙阿萍[2] 宋德懋[3] 

机构地区：[1]北京大学第四临床医院北京积水潭医院检验科,100035 [2]北京大学第三医院神经科 [3]北京大学医学部生理学系

出　　处：《北京医学》2008年第7期424-426,共3页Beijing Medical Journal

摘　　要：目的比较等电聚焦(IEF)与琼脂糖凝胶电泳(AGE)两种检测方法在多发性硬化寡克隆带检测中的差异。方法对45例临床确诊的多发性硬化患者(传统经典型25例与视神经脊髓炎型20例),进行脑脊液和血液的IgG与白蛋白的检测,计算IgG指数。同时使用等电聚焦与凝胶电泳进行脑脊液寡克隆带检测。并对两种方法的敏感性进行比较。结果45例患者中,24例(53%)为OB(IEF)阳性,而只有9例(20%)为OB(AGE)阳性,两者之间有显著性差异(P﹤0.01)。结论等电聚焦法为多发性硬化寡克隆区带较为敏感的检测方法。Objective To compare isoelectric focusing with agarose gel eletrophoresis in detecting oligoclonal band of multiple sclerosis. Methods CSF and sera were collected from 45 patients with clinically definite MS [25 with conventional form of MS （CMS） and 20 with optic-spinal form of MS （OSMS）]. IgG and albumin were tested and IgG index was calculated. The frequency of OB between isoelectric focusing （IEF） and agarose gel electrophoresis （AGE） were compared, Results The frequency of IEF-OB in total MS was 53%, whereas that of AGE-OB was only 20%. The difference was remarkable （P 〈 0.01）. Conclusions Our study strongly suggests that IEF is highly sensitive for detecting OB in patients of multiple sclerosis.

关 键 词：多发性硬化 寡克隆区带 等电聚焦 

分 类 号：R744.51[医药卫生—神经病学与精神病学]