快速液相色谱法测定柑橘中6种类黄酮化合物含量  

Determination of flavonoids in citrus fruit by rapid resolution liquid chromatography

作　　者：张玉[1] 吴慧明[2] 白丽萍[1] 刘晓春[1] 

机构地区：[1]浙江省农业科学院农产品质量标准研究所,杭州310021 [2]浙江大学安全农产品研究中心,杭州310029

出　　处：《果树学报》2008年第4期615-617,共3页Journal of Fruit Science

基　　金：杭州市科技创新项目(20051332B24)

摘　　要：为建立一种快速分析测定柑橘果实中6种类黄酮(橙皮苷、柚皮苷、新橙皮苷、川皮素、桔皮素、橙黄酮)化合物的技术方法,以蜜橘为材料,利用加速溶剂萃取技术对柑橘果实中的6种类黄酮化合物进行提取,并通过快速液相色谱对样品中的类黄酮化合物进行分离测定。结果显示,该方法可以同时测定6种类黄酮化合物,并且具有良好的回收率和精密度。整个提取测定技术快速、简单、准确,适合柑橘果实中6种类黄酮化合物含量的测定。A rapid method was created for determination of 6 flavonoids （naringin, hesperidin, neohesperidin, nobiletin, tangeretin, sinensetin） in citrus fruit. The flavonoids were extracted by ASE from fruit tissue and the concentrations were detected by the rapid resolution liquid chromatography. The results showed the naringin, hesperidin, neohesperidin, nobiletin, tangeretin and sinensetin in citrus fruits could be analyzed simultaneously ; the recovery were 96.5% to 97.7 %and RSD were 1.0% to 1.5%. This method is accurate, rapid and simple and can be used for the determination of flavonoids in citrus fruit.

关 键 词：柑橘 黄酮 快速液相色谱 测定 

分 类 号：S666[农业科学—果树学]